2. Academic Validation
  3. Myogenin suppresses apoptosis induced by angiotensin II in human induced pluripotent stem cell-derived cardiomyocytes

Myogenin suppresses apoptosis induced by angiotensin II in human induced pluripotent stem cell-derived cardiomyocytes

  • Biochem Biophys Res Commun. 2021 May 7;552:84-90. doi: 10.1016/j.bbrc.2021.03.031.
Qiang Gao 1,  Ping Wang 2,  Hailong Qiu 1,  Bin Qiu 3,  Weijin Yi 3,  Wenchang Tu 3,  Bin Lin 4,  Daoheng Sun 3,  Rong Zeng 5,  Meiping Huang 6,  Jimei Chen 1,  Jianzheng Cen 7,  Jian Zhuang 8
Affiliations

Affiliations

  • 1 Department of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, 510100, China.
  • 2 School of Medical Imaging, Tianjin Medical University, Tianjin, 300203, China.
  • 3 Department of Mechanical & Electrical Engineering, Xiamen University, Xiamen, Fujian, 361102, China.
  • 4 Guangdong Beating Origin Regenerative Medicine Co. Ltd., Foshan, Guangdong, 528231, China.
  • 5 Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, 510100, China.
  • 6 Department of Catheterization Lab, Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences Guangzhou, China.
  • 7 Department of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, 510100, China. Electronic address: [email protected].
  • 8 Department of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, 510100, China. Electronic address: [email protected].
PMID: 33743352 DOI: 10.1016/j.bbrc.2021.03.031
Abstract

Background: Angiotensin II (Ang II), an important component of the renin-angiotensin system (Ras), plays a critical role in the pathogenesis of cardiovascular disorders. In addition, human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been considered as a promising platform for studying personalized medicine for heart diseases. However, whether Ang II can induce the Apoptosis of hiPSC-CMs is not known.

Methods: In this study, we treated hiPSC-CMs with different concentrations of Ang II [0 nM (vehicle as a control), 1 nM, 10 nM, 100 nM, 1 μM, 10 μM, 100 μM, and 1 mM] for various time periods (24 h, 48 h, 6 days, and 10 days) and analyzed the viability and Apoptosis of hiPSC-CMs.

Results: We found that treatment with 1 mM Ang II for 10 days reduced the viability of hiPSC-CMs by 41% (p = 2.073E-08) and increased Apoptosis by 2.74-fold, compared to the control group (p = 6.248E-12). MYOG, which encodes the muscle-specific transcription factor myogenin, was also identified as an apoptosis-suppressor gene in Ang II-treated hiPSC-CMs. Ectopic MYOG expression decreased the Apoptosis and increased the viability of Ang II-treated hiPSC-CMs. Further analysis of the RNA sequencing (RNA-seq) data illustrated that myogenin ameliorated Ang II-induced Apoptosis of hiPSC-CMs by downregulating the expression of proinflammatory genes.

Conclusion: Our findings suggest that Ang II induces the Apoptosis of hiPSC-CMs and that myogenin attenuates Ang II-induced Apoptosis.

Keywords

Angiotensin II; Apoptosis; Cell viability; Human induced pluripotent stem cell-derived cardiomyocytes; MYOG.

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