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  3. A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system

A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system

  • STAR Protoc. 2021 Apr 10;2(2):100445. doi: 10.1016/j.xpro.2021.100445.
Lingli He 1,  Wentao Yu 1,  Yi Lu 1,  Wenxiang Zhang 1,  Jinjin Xu 1,  Lei Zhang 1 2 3
Affiliations

Affiliations

  • 1 State Key Laboratory of Cell Biology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.
  • 2 School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • 3 Bio-Research Innovation Center, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Suzhou 215121, China.
PMID: 33912846 DOI: 10.1016/j.xpro.2021.100445
Abstract

Hepatocellular carcinoma (HCC) is the fourth leading cause of Cancer death worldwide. However, the pathogenesis of HCC is complicated, and the drugs used for HCC treatment are limited. The following protocol combines a genetically engineered mouse model (GEMM) with a sleeping beauty system to establish an in vivo liver tumorigenesis model. By using this model, the impact of genes of interest in liver tumorigenesis and progression can be studied. This model can also be applied to develop new therapeutic drugs for HCC. For complete details on the use and execution of this protocol, please refer to He et al. (2020).

Keywords

Cancer; Gene Expression; Genetics; Model Organisms; Molecular Biology.

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