2. Academic Validation
  3. Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury

Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury

  • Nat Commun. 2021 May 17;12(1):2863. doi: 10.1038/s41467-021-23197-7.
Hengwei Jin  # 1,  Kuo Liu  # 1 2,  Juan Tang  # 1,  Xiuzhen Huang 1,  Haixiao Wang 1,  Qianyu Zhang 3,  Huan Zhu 1,  Yan Li 1,  Wenjuan Pu 1,  Huan Zhao 1,  Lingjuan He 1,  Yi Li 1,  Shaohua Zhang 1,  Zhenqian Zhang 1,  Yufei Zhao 4,  Yanqing Qin 5,  Stefan Pflanz 6,  Karim E I Kasmi 6,  Weiyi Zhang 6,  Zhaoyuan Liu 7,  Florent Ginhoux 8,  Yong Ji 9,  Ben He 10,  Lixin Wang 4,  Bin Zhou 11 12 13
Affiliations

Affiliations

  • 1 State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.
  • 2 School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China.
  • 3 School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
  • 4 Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
  • 5 Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, Shanghai, China.
  • 6 Boehringer Ingelheim Pharma GmbH & Co KG, Biberach an der Riss, Germany.
  • 7 Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • 8 Singapore Immunology Network, Agency for Science, Technology and Research, Singapore, Singapore.
  • 9 The Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, Nanjing Medical University, Nanjing, China.
  • 10 Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, China.
  • 11 State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China. [email protected].
  • 12 School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China. [email protected].
  • 13 School of Life Science and Technology, ShanghaiTech University, Shanghai, China. [email protected].
  • # Contributed equally.
PMID: 34001904 DOI: 10.1038/s41467-021-23197-7
Abstract

During injury, monocytes are recruited from the circulation to inflamed tissues and differentiate locally into mature macrophages, with prior reports showing that cavity macrophages of the peritoneum and pericardium invade deeply into the respective organs to promote repair. Here we report a dual recombinase-mediated genetic system designed to trace cavity macrophages in vivo by intersectional detection of two characteristic markers. Lineage tracing with this method shows accumulation of cavity macrophages during lung and liver injury on the surface of visceral organs without penetration into the parenchyma. Additional data suggest that these peritoneal or pleural cavity macrophages do not contribute to tissue repair and regeneration. Our in vivo genetic targeting approach thus provides a reliable method to identify and characterize cavity macrophages during their development and in tissue repair and regeneration, and distinguishes these cells from other lineages.

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