2. Academic Validation
  3. Enhanced Expression but Decreased Specific Activity of Matrix Metalloproteinase 10 (MMP-10) in Comparison with Matrix Metalloproteinase 3 (MMP-3) in Human Urinary Bladder Carcinoma

Enhanced Expression but Decreased Specific Activity of Matrix Metalloproteinase 10 (MMP-10) in Comparison with Matrix Metalloproteinase 3 (MMP-3) in Human Urinary Bladder Carcinoma

  • J Clin Med. 2021 Aug 19;10(16):3683. doi: 10.3390/jcm10163683.
Jacek Kudelski 1,  Grzegorz Młynarczyk 1 2,  Monika Gudowska-Sawczuk 3,  Barbara Mroczko 3 4,  Barbara Darewicz 1,  Marta Bruczko-Goralewska 2,  Krzysztof Sobolewski 2,  Lech Romanowicz 2
Affiliations

Affiliations

  • 1 Department of Urology, Medical University of Bialystok, M. Skłodowskiej-Curie 24A St., 15-276 Białystok, Poland.
  • 2 Department of Medical Biochemistry, Medical University of Bialystok, Adama Mickiewicza 2C St., 15-089 Białystok, Poland.
  • 3 Department of Biochemical Diagnostics, Medical University of Bialystok, Waszyngtona 15A St., 15-269 Bialystok, Poland.
  • 4 Department of Neurodegeneration Diagnostics, Medical University of Bialystok, Waszyngtona 15A St., 15-269 Bialystok, Poland.
PMID: 34441979 DOI: 10.3390/jcm10163683
Abstract

Human urinary bladder Cancer is a huge worldwide oncological problem causing many deaths every year. The degradation of extracellular matrix (ECM) induced by molecules such as Matrix Metalloproteinases (MMPs) is one of the main factors influencing the process of metastasis origination. The MMP expression is tied to tumor aggressiveness, stage, and patient prognosis. The cleavage of constituent proteins is initiated and prolonged by Matrix Metalloproteinases, such as MMP-3 and MMP-10. The aim of this study was to evaluate the expression and activity of both MMPs in human urinary bladder Cancer occurring at various stages of the disease. Tissue samples from patients with urinary bladder Cancer were analyzed. Samples were collected from patients with a low- and high-grade Cancer. Control tissue was collected from the site opposite to the tumor. DNA content, MMPs content, and activity of MMP-3 and MMP-10 were measured using ELISA and Western blot techniques. MMP-3 and MMP-10 occur in high molecular complexes in human urinary bladder in healthy and cancerous tissues. Particularly, in high-grade tumors, the content of MMP-10 prevails over MMP-3. The actual and specific activities vary in both grades of urinary bladder cancer; however, the highest activity for MMP-3 and MMP-10 was found in low-grade tissues. In conclusion, MMP-10 had a higher content, but a lower activity in all investigated tissues compared to MMP-3. Generally, obtained results demonstrated a contrary participation of MMP-3 and MMP-10 in ECM remodeling what may be crucial in the pathogenesis of human urinary bladder carcinoma.

Keywords

MMP-10; MMP-3; biomarker; cancer; urinary bladder carcinoma.

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