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  3. Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity

Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity

  • STAR Protoc. 2022 Mar 4;3(1):101218. doi: 10.1016/j.xpro.2022.101218.
Christopher J Bergin 1,  Yannick D Benoit 1
Affiliations

Affiliation

  • 1 Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
PMID: 35265864 DOI: 10.1016/j.xpro.2022.101218
Abstract

Organoids can enable the study of solid tumors initiated from a single Cancer stem cell (CSC) ex vivo. We describe a serial tumor Organoid plating protocol using primary colorectal Cancer (CRC) tissues as a rapid and cost-efficient approach to evaluate the impact of therapeutic interventions on CSC functions. We detail the isolation of primary colorectal CSCs, Organoid embedding, serial passaging, and CSC-related analytical techniques. For complete details on the use and execution of this protocol, please refer to Masibag et al. (2021) and Bergin et al. (2021).

Keywords

Cancer; Cell isolation; Cell-based Assays; Health Sciences; Microscopy; Organoids; Single Cell; Stem Cells; Tissue Engineering.

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