2. Academic Validation
  3. Transcriptome-wide identification of microRNAs in response to 20-hydroxyecdysone in Galeruca daurica

Transcriptome-wide identification of microRNAs in response to 20-hydroxyecdysone in Galeruca daurica

  • Comp Biochem Physiol Part D Genomics Proteomics. 2022 Jun:42:100981. doi: 10.1016/j.cbd.2022.100981.
Hai-Chao Wang 1,  Hai-Bin Han 2,  Tian-Feng Duan 1,  Ling Li 1,  Bao-Ping Pang 3
Affiliations

Affiliations

  • 1 Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot 010020, China.
  • 2 Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010020, China.
  • 3 Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot 010020, China. Electronic address: [email protected].
PMID: 35276449 DOI: 10.1016/j.cbd.2022.100981
Abstract

Both 20-hydroxyecdysone (20E) and miRNAs have multiple functions in the regulation of various physiological processes in insects. However, little is known about the interaction between 20E and miRNAs. In this study, six small RNA libraries were constructed from the adult Galeruca daurica treated with 20E and dimethyl sulfoxide (DMSO), respectively. Using small RNA Sequencing, a total of 183 miRNAs, including 140 known and 43 novel miRNAs, were identified. Compared with the control (DMSO), 52 miRNAs (21 up-regulated and 31 down-regulated) were significantly differentially expressed after 20E treatment. The KEGG and GO analysis of the predicted genes targeted by 20E-responsive miRNAs indicate that 20E may influence the metabolic change during reproductive diapause in G. daurica via regulating miRNAs.

Keywords

20-hydroxyecdysone; Galeruca daurica; MicroRNA; Small RNA sequencing.

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