2. Academic Validation
  3. Hawthorn extract inhibited the PI3k/Akt pathway to prolong the lifespan of Drosophila melanogaster

Hawthorn extract inhibited the PI3k/Akt pathway to prolong the lifespan of Drosophila melanogaster

  • J Food Biochem. 2022 Aug;46(8):e14169. doi: 10.1111/jfbc.14169.
Yichun Wang 1,  Huali Wang 2,  Tianjiao Ma 3,  Guishan Liu 4,  Xu Feng 5,  Xiaozhi Liu 6 7,  Xiaofang Ma 7,  Suwen Liu 8,  Donglin Shi 9,  Biao Wang 10,  Ji Kang 1,  Hao Wang 1,  Zhiwei Wang 1
Affiliations

Affiliations

  • 1 State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin, China.
  • 2 China National Center for Food Safety Risk Assessment, Beijing, China.
  • 3 Institute of Quartermaster Engineering and Technology, Academy of Military Sciences PLA China, Beijing, China.
  • 4 School of Food & Wine, Ningxia University, Yinchuan, China.
  • 5 Naval Medical Research Institute, Second Military Medical University, Shanghai, China.
  • 6 Tianjin Key Laboratory of Epigenetics for Organ Development in Preterm Infants, The Fifth Central Hospital of Tianjin, Tianjin, China.
  • 7 Department of Neurosurgery, The Fifth Central Hospital of Tianjin, Tianjin, China.
  • 8 College of Food Science & Technology, Hebei Normal University of Science and Technology, Qinhuangdao, China.
  • 9 Department of Physical Education, Hebei Sport University, Shijiazhuang, China.
  • 10 College of Chemical Engineering and Material Science, Tianjin University of Science and Technology, Tianjin, China.
PMID: 35383968 DOI: 10.1111/jfbc.14169
Abstract

HE is a natural extract with strong antioxidant capacity. Drosophila melanogaster was used to explore HE could delay aging in this study. We detected that 3 mg/ml HE could increase stress tolerance (heat, cold, starvation, oxidative stress), reduce intestinal dysfunction, and prolong the lifespan of D. melanogaster. Network pharmacology analysis showed HE could act through the PI3K-Akt pathway. Meanwhile, HE intervention inhibited the gene expression of InR, PI3K, and Akt-1, and further increased the gene expression of Atg1, Atg5, Atg8a, and Atg8b. Furthermore, HE inhibited the unnatural propagation of ISCs and increased the number of lysosomes. Supplement with HE may be an effective intervention for aging D. melanogaster. PRACTICAL APPLICATIONS: In recent years, diseases that come with aging have seriously affected people's healthy life. Hawthorn is a kind of nutrient-rich substance that is rich in Flavonoids and thus has many potential biological and pharmacological functions. Our results showed that HE has good antioxidant properties and can maintain intestinal homeostasis, which provides a good theoretical basis for the development and research using HE as an effective natural antioxidant for the elderly.

Keywords

Drosophila melanogaster; PI3K-Akt pathway; autophagy; hawthorn extract; lifespan.

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