1. Academic Validation
  2. Sparstolonin B inhibits pancreatic adenocarcinoma through the NF-κB signaling pathway

Sparstolonin B inhibits pancreatic adenocarcinoma through the NF-κB signaling pathway

  • Exp Cell Res. 2022 Aug 1;417(1):113214. doi: 10.1016/j.yexcr.2022.113214.
Yang Lyu 1 Bowen Duan 1 Ziming Liu 1 Fan Yang 1 Chen Chen 1 Xuejiao Jiang 1 Xiang Liu 2
Affiliations

Affiliations

  • 1 Department of Gastroenterology, Shengjing Hospital of China Medical University, NO.36, Sanhao Street, Shenyang, Liaoning Province, 110004, PR China.
  • 2 Department of Gastroenterology, Shengjing Hospital of China Medical University, NO.36, Sanhao Street, Shenyang, Liaoning Province, 110004, PR China. Electronic address: [email protected].
Abstract

Pancreatic adenocarcinoma is a highly lethal malignant gastrointestinal tumor. Sparstolonin B is an isocoumarin whose Anticancer activity has recently received increasing attention. This study aimed to investigate Sparstolonin B's potential antitumor effect on pancreatic adenocarcinoma. The effect of Sparstolonin B on pancreatic Cancer target genes and molecular mechanism was predicted via network pharmacology; Sparstolonin B significantly decreased Panc-1 and SW1990 cell viability and effectively suppressed the proliferation, migration, and invasion of pancreatic Cancer cells as shown by CCK-8, colony formation, and Transwell assays. Flow cytometry showed that it induced cell cycle arrest and Apoptosis. Sparstolonin B also upregulated Bax levels but decreased those of MMP2 and Bcl-2, downregulated IκBα expression, and upregulated p65 and IκBα phosphorylation; however, it had no effect on total NF-κB p65 levels. The NF-κB pathway inhibitor QNZ reversed these effects. The treatment group (26 μmol/L) had reduced graft volume and weight and fewer Ki-67-positive cells than the control group. Therefore, Sparstolonin B can inhibit the growth and induce the Apoptosis of pancreatic Cancer cells via the NF-κB signaling pathway and may be a potential novel drug for pancreatic Cancer treatment.

Keywords

Apoptosis; Nuclear factor (NF)-Kappa B; Pancreatic adenocarcinoma; Sparstolonin B.

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