1. NF-κB
    Apoptosis
  2. NF-κB
    TNF Receptor
  3. QNZ

QNZ (Synonyms: EVP4593)

Cat. No.: HY-13812 Purity: 98.46%
Handling Instructions

QNZ (EVP4593) shows strong inhibitory effects on NF-κB transcriptional activation and TNF-α production with IC50s of 11 and 7 nM, respectively. QNZ (EVP4593) is a neuroprotective inhibitor of SOC channel.

For research use only. We do not sell to patients.

QNZ Chemical Structure

QNZ Chemical Structure

CAS No. : 545380-34-5

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 106 In-stock
Estimated Time of Arrival: December 31
5 mg USD 96 In-stock
Estimated Time of Arrival: December 31
10 mg USD 180 In-stock
Estimated Time of Arrival: December 31
50 mg USD 516 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 10 publication(s) in Google Scholar

Top Publications Citing Use of Products

    QNZ purchased from MCE. Usage Cited in: J Cell Mol Med. 2018 Dec;22(12):6039-6054.

    NF-κB protein expression in cells treated with Tp92 (5 μg/mL) or Tp92 (5 μg/mL)+QNZ (10 nM).

    QNZ purchased from MCE. Usage Cited in: J Mol Cell Biol. 2018 Oct 15. 

    Cytoplasmic LPS-induced GSDMB and GSDMD expression are both significantly attenuated by either JSH-23 or QNZ.

    QNZ purchased from MCE. Usage Cited in: Clin Sci (Lond). 2019 Jul 15;133(13):1523-1536.

    Western blot analyses show that the inhibition of NF-κB/p65 enhanced the protective effect of ergosterol in CSE-induced 16HBE cells.

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    Description

    QNZ (EVP4593) shows strong inhibitory effects on NF-κB transcriptional activation and TNF-α production with IC50s of 11 and 7 nM, respectively. QNZ (EVP4593) is a neuroprotective inhibitor of SOC channel.

    IC50 & Target[1]

    NF-κB

    11 nM (IC50, in human Jurkat cells transfected with pNFκB-Luc)

    TNF-α

    7 nM (IC50, in murine splenocytes stimulated with LPS)

    In Vitro

    QNZ (Compound 11q) has a suppressing effect of the NF-κB mediated-inflammatory response. QNZ inhibits edema formation dose-dependently[1]. QNZ (EVP4593) reduces the number of lysosomes/autophagosomes and store-operated channel (SOC) currents in Huntington's disease (HD). Normalization of calcium transport within neurons in response to QNZ is expect to reduce pathology manifestation. A number of lysosomes/autophagosomes are evaluated in HD and WT neurons treated with QNZ using transmission electron microscopy (TEM). Incubation with QNZ reduces the number of lysosomes/autophagosomes in HD GABAergic medium spiny (GABA MS)-like neurons (GMSLNs) by almost two-fold (from 0.41±0.04 to 0.23±0.04; p<0.05), while WT neurons are not affected. This observation is confirmed by examining lysosome content by flow cytometry (FC) analysis. The median fluorescence intensity is reduced by 34±6 % in HD GMSLNs upon QNZ treatment (p<0.05)[2].

    Molecular Weight

    356.42

    Formula

    C₂₂H₂₀N₄O

    CAS No.

    545380-34-5

    SMILES

    NC1=CC2=C(NCCC3=CC=C(OC4=CC=CC=C4)C=C3)N=CN=C2C=C1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 37 mg/mL (103.81 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8057 mL 14.0284 mL 28.0568 mL
    5 mM 0.5611 mL 2.8057 mL 5.6114 mL
    10 mM 0.2806 mL 1.4028 mL 2.8057 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (7.01 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (7.01 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (7.01 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [2]

    iPSHD22 cells are cultured in K-4 medium in a 96-well black plates with clear flat bottom. Next, cells are treated with chemical compounds (e.g., QNZ 100 nM) for 24 h prior to analysis. Fluorescent assay MultiTox-Fluor Multiplex Cytotoxicity Assay is used to measure simultaneously the relative number of live (viability) and dead (cytotoxicity) cells in each well. Fluorescence is detected by DTX 880 Multimode Microplate Reader. To evaluate the level of cell death (LoCD), the following equation is employed: ([cytotoxicity in a well with cells]-[cytotoxicity in a well without cells])/([viability in a well with cells]-[viability in a well without cells])[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    QNZEVP4593EVP 4593EVP-4593NF-κBTNF ReceptorNuclear factor-κBNuclear factor-kappaBTumor Necrosis Factor ReceptorTNFRInhibitorinhibitorinhibit

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    Product name:
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    Cat. No.:
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