2. NF-κB Anti-infection
  3. NF-κB Influenza Virus
  4. SC75741

SC75741 est un inhibiteur de NF-κB qui est large et efficace avec un IC50 de 200 nM pour p65. SC75741 bloque la réplication des virus grippaux (IV). SC75741 altère la liaison à l'ADN de la sous-unité p65 NF-κB, entraînant une expression réduite des cytokines, des chimiokines et des facteurs pro-apoptotiques. SC75741 inhibe ensuite l'activation de la caspase et bloque l'exportation nucléaire de la caspase-médiée des ribonucléoprotéines virales.

SC75741 is a broad and efficient NF-κB inhibitor with an IC50 of 200 nM for p65. SC75741 blocks influenza viruses (IV) replication. SC75741 impairs DNA binding of the NF-κB subunit p65, resulting in reduced expression of cytokines, chemokines, and pro-apoptotic factors. SC75741 subsequently inhibits caspase activation and blocks caspase-mediated nuclear export of viral ribonucleoproteins.

For research use only. We do not sell to patients.

SC75741 Chemical Structure

SC75741 Chemical Structure

CAS No. : 913822-46-5

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 110 In-stock
Solution
10 mM * 1 mL in DMSO USD 110 In-stock
Solid
1 mg USD 47 In-stock
5 mg USD 88 In-stock
10 mg USD 150 In-stock
25 mg USD 300 In-stock
50 mg USD 480 In-stock
100 mg USD 720 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 11 publication(s) in Google Scholar

SC75741 Related Antibodies

Top Publications Citing Use of Products

View All NF-κB Isoform Specific Products:

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SC75741 is a broad and efficient NF-κB inhibitor with an IC50 of 200 nM for p65[1]. SC75741 blocks influenza viruses (IV) replication. SC75741 impairs DNA binding of the NF-κB subunit p65, resulting in reduced expression of cytokines, chemokines, and pro-apoptotic factors. SC75741 subsequently inhibits caspase activation and blocks caspase-mediated nuclear export of viral ribonucleoproteins[2].

IC50 & Target[1,2]

p65

200 nM (IC50)

In Vitro

SC75741 (5 μM; 24-96 hours) inhibits long-term A549 cells proliferation[2].
SC75741 (1-10 μM; 5.5-65 hours) reduces A549 cells viability in a concentration-dependent manner indicating a cytostatic effect for A549 cells within a time frame of about 50 and 65 hours[2].
SC75741 (5 μM; 24 hours) strongly inhibits cleavage of the effector caspase 3 induced upon H7N7-infection[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SC75741 Related Antibodies

Cell Proliferation Assay[2]

Cell Line: A549 cells
Concentration: 5 μM
Incubation Time: 24, 48, 72 and 96 hours
Result: Inhibited long-term cell proliferation

Cell Viability Assay[2]

Cell Line: A549 cells
Concentration: 1, 2, 5 or 10 μM
Incubation Time: 5.5, 29, 50, 65 hours
Result: Reduced cells viability in a concentration-dependent manner.

Western Blot Analysis[2]

Cell Line: MDCK cells
Concentration: 5 μM
Incubation Time: 24 hours
Result: Inhibited cleavage of the effector caspase 3 induced upon H7N7-infection.
In Vivo

SC75741 (intraperitoneal injection; 15 mg/kg; for 2 days) leads to a reduced propagation of the H5N1 virus mRNA by 90% in the lungs of infected mice[2].
The plasma-levels of SC74751 (intravenously of 5 mg/kg and intraperitoneally of 15 mg/kg; for 3.5 and 6 hours) after i.v. administration decreases mono-exponentially and half-life is roughly 40 min. After i.p. administration, elimination of SC75741 seems to be limited by a slow uptake from the peritoneum and a half-life of 55 min is observed[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Inbred female C57BL/6 mice at the age of 6-8 weeks[2]
Dosage: 15 mg/kg
Administration: Intraperitoneal injection; for 2 days
Result: Reduced the amount of viral mRNA by 90%.
Animal Model: Inbred female C57BL/6 mice at the age of 6-8 weeks[1]
Dosage: 5 mg/kg or 15 mg/kg
Administration: Intravenously of 5 mg/kg and intraperitoneally of 15 mg/kg; 3.5 and 6 hours
Result: Half-life was roughly 40 min and 55 min for i.v. and i.p. administration, respectively.
Molecular Weight

565.67

Formula

C29H23N7O2S2
CAS No.
Appearance

Solid

Color

Light yellow to brown

SMILES

O=C(NC1=NC(C=CC(C(C2=CC=CC=C2)=O)=C3)=C3N1)C4=CSC(C(CC5)CCN5C6=NC=NC7=C6SC=C7)=N4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (147.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7678 mL 8.8391 mL 17.6782 mL
5 mM 0.3536 mL 1.7678 mL 3.5356 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (3.68 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (3.68 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.51%

COA (249 KB) HNMR (218 KB) RP-HPLC (243 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7678 mL 8.8391 mL 17.6782 mL 44.1954 mL
5 mM 0.3536 mL 1.7678 mL 3.5356 mL 8.8391 mL
10 mM 0.1768 mL 0.8839 mL 1.7678 mL 4.4195 mL
15 mM 0.1179 mL 0.5893 mL 1.1785 mL 2.9464 mL
20 mM 0.0884 mL 0.4420 mL 0.8839 mL 2.2098 mL
25 mM 0.0707 mL 0.3536 mL 0.7071 mL 1.7678 mL
30 mM 0.0589 mL 0.2946 mL 0.5893 mL 1.4732 mL
40 mM 0.0442 mL 0.2210 mL 0.4420 mL 1.1049 mL
50 mM 0.0354 mL 0.1768 mL 0.3536 mL 0.8839 mL
60 mM 0.0295 mL 0.1473 mL 0.2946 mL 0.7366 mL
80 mM 0.0221 mL 0.1105 mL 0.2210 mL 0.5524 mL
100 mM 0.0177 mL 0.0884 mL 0.1768 mL 0.4420 mL
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SC75741 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SC75741913822-46-5SC 75741SC-75741NF-κBInfluenza VirusNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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