SC75741

Name: SC75741
Brand: MedChemExpress
SKU: HY-10496
Rating: 5.0 (32 reviews)

Cat. No.: HY-10496 Purity: 99.24%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

SC75741 is a broad and efficient NF-κB inhibitor with an IC₅₀ of 200 nM for p65. SC75741 blocks influenza viruses (IV) replication. SC75741 impairs DNA binding of the NF-κB subunit p65, resulting in reduced expression of cytokines, chemokines, and pro-apoptotic factors. SC75741 subsequently inhibits caspase activation and blocks caspase-mediated nuclear export of viral ribonucleoproteins.

For research use only. We do not sell to patients.

CAS No. : 913822-46-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 32 publication(s) in Google Scholar

Other Forms of SC75741:

SC75741 (Standard) Get quote

32 Publications Citing Use of MCE SC75741

RT-PCR

Cell Imaging/Staining

Cell Proliferation/Viability Assay

: SC75741 purchased from MedChemExpress. Usage Cited in: Sci Bull. 2025 May 30;70(10):1673-1690.; SC75741 (5 μmol/L). Western blot analysis showed that SC75741 treatment significantly downregulated the expression of p-p65 (Ser529).

: SC75741 purchased from MedChemExpress. Usage Cited in: Sci Bull. 2025 May 30;70(10):1673-1690.; SC75741 (5 μmol/L). The fluorescence staining confirmed NFκB inhibition led to less myocardial apoptosis and ROS production after 8-hours SCS.

: SC75741 purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Oct 1;134(22):e178628. [Abstract]; Relative mRNA expression of OLFML3 in PTEN-KO SF763 cells expressing shC and shLOX treated with or without P65 inhibitor (P65i) SC75741 (5 μM, 24h).

: SC75741 purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Oct 1;134(22):e178628. [Abstract]; Immunoblots for OLFML3 in lysates of PTEN-WT SF763 cells expressing shC and shPATZ1 treated with or without P65i SC75741 (5 μM, 24h).

: SC75741 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2024 May 29:101592. [Abstract]; MV4-11 cells stimulated in LDM supplied with different reagents (PA, 20 μM; OxLDL, 25 μg/mL; SC75741, 5 μM) for 3 days were further used for T cell proliferation assay (n = 3).

: SC75741 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2024 May 29:101592. [Abstract]; Different kinds of MV4-11 cells were used for T cell proliferation assay (n = 3). SC75741 (5 μM) was added.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

SC75741 is a broad and efficient NF-κB inhibitor with an IC₅₀ of 200 nM for p65^[1]. SC75741 blocks influenza viruses (IV) replication. SC75741 impairs DNA binding of the NF-κB subunit p65, resulting in reduced expression of cytokines, chemokines, and pro-apoptotic factors. SC75741 subsequently inhibits caspase activation and blocks caspase-mediated nuclear export of viral ribonucleoproteins^[2].

IC₅₀ & Target^[1,2]

p65

200 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	0.2 μM Compound: 13	Inhibition of NF-kappaB in A549 cells after 5 hrs by reporter gene assay Inhibition of NF-kappaB in A549 cells after 5 hrs by reporter gene assay	[PMID: 17869512]
PBMC	IC₅₀	2.2 μM Compound: 13	Antiproliferative activity against human PBMC Antiproliferative activity against human PBMC	[PMID: 17869512]
THP1-Dual	IC₅₀	3958 nM Compound: SC75741	Inhibition of LPS induced ISG activation in human THP1-Dual cells pretreated with LPS-B5 for 3 hrs followed by compound addition incubated for 24 hrs Inhibition of LPS induced ISG activation in human THP1-Dual cells pretreated with LPS-B5 for 3 hrs followed by compound addition incubated for 24 hrs	[PMID: 36053746]
THP1-Dual	IC₅₀	5975 nM Compound: SC75741	Inhibition of LPS induced NF kappa B activation in human THP1-Dual cells stimulated with LPS-B5 for 3 hrs followed by compound addition incubated for 24 hrs Inhibition of LPS induced NF kappa B activation in human THP1-Dual cells stimulated with LPS-B5 for 3 hrs followed by compound addition incubated for 24 hrs	[PMID: 36053746]

In Vitro

SC75741 (5 μM; 24-96 hours) inhibits long-term A549 cells proliferation^[2].
SC75741 (1-10 μM; 5.5-65 hours) reduces A549 cells viability in a concentration-dependent manner indicating a cytostatic effect for A549 cells within a time frame of about 50 and 65 hours^[2].
SC75741 (5 μM; 24 hours) strongly inhibits cleavage of the effector caspase 3 induced upon H7N7-infection^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	A549 cells
Concentration:	5 μM
Incubation Time:	24, 48, 72 and 96 hours
Result:	Inhibited long-term cell proliferation

Cell Viability Assay^[2]

Cell Line:	A549 cells
Concentration:	1, 2, 5 or 10 μM
Incubation Time:	5.5, 29, 50, 65 hours
Result:	Reduced cells viability in a concentration-dependent manner.

Western Blot Analysis^[2]

Cell Line:	MDCK cells
Concentration:	5 μM
Incubation Time:	24 hours
Result:	Inhibited cleavage of the effector caspase 3 induced upon H7N7-infection.

In Vivo

SC75741 (intraperitoneal injection; 15 mg/kg; for 2 days) leads to a reduced propagation of the H5N1 virus mRNA by 90% in the lungs of infected mice^[2].
The plasma-levels of SC74751 (intravenously of 5 mg/kg and intraperitoneally of 15 mg/kg; for 3.5 and 6 hours) after i.v. administration decreases mono-exponentially and half-life is roughly 40 min. After i.p. administration, elimination of SC75741 seems to be limited by a slow uptake from the peritoneum and a half-life of 55 min is observed^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Inbred female C57BL/6 mice at the age of 6-8 weeks^[2]
Dosage:	15 mg/kg
Administration:	Intraperitoneal injection; for 2 days
Result:	Reduced the amount of viral mRNA by 90%.

Animal Model:	Inbred female C57BL/6 mice at the age of 6-8 weeks^[1]
Dosage:	5 mg/kg or 15 mg/kg
Administration:	Intravenously of 5 mg/kg and intraperitoneally of 15 mg/kg; 3.5 and 6 hours
Result:	Half-life was roughly 40 min and 55 min for i.v. and i.p. administration, respectively.

Molecular Weight

565.67

Formula

C₂₉H₂₃N₇O₂S₂

CAS No.

913822-46-5

Appearance

Solid

Color

Light yellow to brown

SMILES

O=C(NC1=NC(C=CC(C(C2=CC=CC=C2)=O)=C3)=C3N1)C4=CSC(C(CC5)CCN5C6=NC=NC7=C6SC=C7)=N4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (147.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7678 mL	8.8391 mL	17.6782 mL
5 mM	0.3536 mL	1.7678 mL	3.5356 mL
10 mM	0.1768 mL	0.8839 mL	1.7678 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (3.68 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.24%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (249 KB) HNMR (309 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Haasbach E, et al. The NF-kappaB inhibitor SC75741 protects mice against highly pathogenic avian influenza A virus. Antiviral Res. 2013 Sep;99(3):336-44. [Content Brief]

[2]. Ehrhardt C, et al. The NF-κB inhibitor SC75741 efficiently blocks influenza virus propagation and confers a high barrier for development of viral resistance. Cell Microbiol. 2013 Jul;15(7):1198-211. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7678 mL	8.8391 mL	17.6782 mL	44.1954 mL
	5 mM	0.3536 mL	1.7678 mL	3.5356 mL	8.8391 mL
	10 mM	0.1768 mL	0.8839 mL	1.7678 mL	4.4195 mL
	15 mM	0.1179 mL	0.5893 mL	1.1785 mL	2.9464 mL
	20 mM	0.0884 mL	0.4420 mL	0.8839 mL	2.2098 mL
	25 mM	0.0707 mL	0.3536 mL	0.7071 mL	1.7678 mL
	30 mM	0.0589 mL	0.2946 mL	0.5893 mL	1.4732 mL
	40 mM	0.0442 mL	0.2210 mL	0.4420 mL	1.1049 mL
	50 mM	0.0354 mL	0.1768 mL	0.3536 mL	0.8839 mL
	60 mM	0.0295 mL	0.1473 mL	0.2946 mL	0.7366 mL
	80 mM	0.0221 mL	0.1105 mL	0.2210 mL	0.5524 mL
	100 mM	0.0177 mL	0.0884 mL	0.1768 mL	0.4420 mL

SC75741 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SC75741913822-46-5SC 75741SC-75741NF-κBInfluenza VirusNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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SC75741

Customer Review

Other Forms of SC75741:

SC75741 Related Antibodies

32 Publications Citing Use of MCE SC75741

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All NF-κB Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

SC75741 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

SC75741 Related Classifications

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