Sparstolonin B

Name: Sparstolonin B
Brand: MedChemExpress
SKU: HY-116213
Rating: 5.0 (9 reviews)

Cat. No.: HY-116213 Purity: 99.94%: Data Sheet
COA

SDS
Handling Instructions
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Sparstolonin B acts as a selective TLR2 and TLR4 antagonist and selectively blocks TLR2- and TLR4-mediated inflammatory signaling. Sparstolonin B has anti-HIV and anticancer activities.

For research use only. We do not sell to patients.

CAS No. : 1259330-61-4

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 9 publication(s) in Google Scholar

9 Publications Citing Use of MCE Sparstolonin B

Others

Cell Migration/Invasion Assay

Flow Cytometry

RT-PCR

: Sparstolonin B purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Sep 30;44(1):277. [Abstract]; Rescue experiments assessing the effect of adding recombinant SAA1 protein (HY-P70510; 200 ng/ml; ; 20–24 h) or the TLR2/4 inhibitor SsnB (Sparstolonin B; HY-116213; 20 µM; 20–24 h) on the ability of A2780 cell supernatants to recruit MDSCs.

: Sparstolonin B purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Sep 30;44(1):277. [Abstract]; Rescue experiments assessing the effect of adding recombinant SAA1 protein (HY-P70510; 200 ng/ml; ; 20–24 h) or the TLR2/4 inhibitor SsnB (Sparstolonin B; HY-116213; 20 µM; 20–24 h) on the ability of A2780 cell supernatants to recruit MDSCs.

: Sparstolonin B purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Sep 30;44(1):277. [Abstract]; Rescue experiments assessing the effect of recombinant SAA1 protein (HY-P700309; 200 ng/ml; ; 20–24 h) or SsnB (Sparstolonin B; HY-116213; 20 µM; 20–24 h) on the ability of ID8 cell supernatants to recruit MDSCs. Data are presented as mean ± SEM from three independent experiments; one-way ANOVA followed by Tukey’s multiple comparisons test.

: Sparstolonin B purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Sep 30;44(1):277. [Abstract]; Rescue experiments assessing the effect of adding recombinant SAA1 protein (HY-P70510; 200 ng/ml; ; 20–24 h) or the TLR2/4 inhibitor SsnB (Sparstolonin B; HY-116213; 20 µM; 20–24 h) on the ability of A2780 cell supernatants to induce GMP differentiation into MDSCs. Data are presented as mean ± SEM from three independent experiments; one-way ANOVA followed by Tukey’s multiple comparisons test.

: Sparstolonin B purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Sep 30;44(1):277. [Abstract]; IL-1β expression and secretion in MDSCs after adding the TLR2/4 inhibitor SsnB (Sparstolonin B; HY-116213; 20 µM; 20–24 h) to SAA1 (HY-P70510; 200 ng/ml; ; 20–24 h) treatment, detected by qRT-PCR (left) and ELISA (right). Data are presented as mean ± SEM from three independent experiments; one-way ANOVA followed by Dunnett’s multiple comparisons test.

: Sparstolonin B purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Aug 26;23(1):381. [Abstract]; Neutrophils were pretreated with the TLR2 and TLR4 inhibitor Sparstolonin B (SsnB) (HY-116213; 10 µM), the TLR2 inhibitor C29 (HY-100461; 100 µM), or the TLR4 inhibitor C34 (TLR4-IN-C34; HY-107575; 10 µM) for 30 min before stimulation with calreticulin. Transwell migration assay. The lower chambers were loaded with blank culture medium (a), calreticulin (b), calreticulin with SsnB (c), calreticulin with C29 (d), or calreticulin with C34 (e). CFSE-labeled neutrophils were loaded into the upper chamber and allowed to migrate for 3 h.

: Sparstolonin B purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Aug 26;23(1):381. [Abstract]; Neutrophils were pretreated with the TLR2 and TLR4 inhibitor Sparstolonin B (SsnB) (HY-116213; 10 µM), the TLR2 inhibitor C29 (HY-100461; 100 µM), or the TLR4 inhibitor C34 (TLR4-IN-C34; HY-107575; 10 µM) for 30 min before stimulation with calreticulin. F-actin was detected for 30 min the incubation of neutrophils with calreticulin in the presence of the respective inhibitors (scale bars = 10 μm).

: Sparstolonin B purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Aug 26;23(1):381. [Abstract]; Neutrophils were incubated in calreticulin with or without inhibitors of TLR2/TLR4 (SsnB; HY-116213; 10 µM), TLR2 (C29; HY-100461; 100 µM), or TLR4 (C34; TLR4-IN-C34; HY-107575; 10 µM) for 0 min, 1 min, 3 min, or 5 min. The cell lysate was prepared and subjected to western blotting for analysis of the expression of Cdc42, Rac1, and RhoA. Densitometric analysis of each protein band is shown below the blots, representing grayscale quantification relative to internal controls

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Sparstolonin B acts as a selective TLR2 and TLR4 antagonist and selectively blocks TLR2- and TLR4-mediated inflammatory signaling. Sparstolonin B has anti-HIV and anticancer activities^[1]^[2].

IC₅₀ & Target^[1]^[2]

TLR2

TLR4

HIV-1

In Vitro

Sparstolonin B (1-20 μM; 2-4 days) inhibits cell growth and viability of neuroblastoma cells^[3].
Sparstolonin B inhibits TLR ligand-induced cytokine expression in mouse macrophages. Sparstolonin B inhibits MyD88 recruitment to TLR4 and TLR2^[1].
Sparstolonin B generates reactive oxygen species (ROS) in neuroblastoma cells. Sparstolonin B reduces expression of N-myc in neuroblastoma cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	SH-SY5Y, IMR-32, NGP, SKNF-1 and SK-N-BE(2) cells
Concentration:	1 µM, 5 µM, 10 µM or 20 µM
Incubation Time:	2-4 days
Result:	Effectively and dose-dependently inhibits the viability of all neuroblastoma cell lines after 2 days (SH-SY5Y and IMR-32), 3 days (NGP cells) or 4 days (SKNF-1 and SK-N-BE(2) cells) treatment.

In Vivo

Sparstolonin B (100 μg/mouse; i.p.) suppresses LPS-provoked inflammation in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	5-6-week-old male C57Bl/6 mice (body weight 18-20 g)^[1]
Dosage:	100 μg/mouse
Administration:	I.p.
Result:	Significantly lower TNFα and IL-1β expression levels in LPS-induced sepsis mouse model.

Molecular Weight

268.22

Formula

C₁₅H₈O₅

CAS No.

1259330-61-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1OC=C2C3=C(OC4=C2C1=C(O)C=C4)C=CC(O)=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (37.28 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7283 mL	18.6414 mL	37.2828 mL
5 mM	0.7457 mL	3.7283 mL	7.4566 mL
10 mM	0.3728 mL	1.8641 mL	3.7283 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 5 mg/mL (18.64 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (276 KB) SDS (392 KB)

COA (249 KB) HNMR (163 KB) RP-HPLC (245 KB)

Handling Instructions (2659 KB)

References

[1]. Liang Q, et al. Characterization of sparstolonin B, a Chinese herb-derived compound, as a selective Toll-like receptor antagonist with potent anti-inflammatory properties. J Biol Chem. 2011;286(30):26470-26479. [Content Brief]

[2]. Deng X, et al. The Chinese herb-derived Sparstolonin B suppresses HIV-1 transcription. Virol J. 2015;12:108. Published 2015 Jul 25. [Content Brief]

[3]. Kumar A, et al. Sparstolonin B, a novel plant derived compound, arrests cell cycle and induces apoptosis in N-myc amplified and N-myc nonamplified neuroblastoma cells [published correction appears in PLoS One. 2016;11(7):e0159082]. PLoS One. 2014;9(5):e96343. Published 2014 May 1. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7283 mL	18.6414 mL	37.2828 mL	93.2071 mL
	5 mM	0.7457 mL	3.7283 mL	7.4566 mL	18.6414 mL
	10 mM	0.3728 mL	1.8641 mL	3.7283 mL	9.3207 mL
	15 mM	0.2486 mL	1.2428 mL	2.4855 mL	6.2138 mL
	20 mM	0.1864 mL	0.9321 mL	1.8641 mL	4.6604 mL
	25 mM	0.1491 mL	0.7457 mL	1.4913 mL	3.7283 mL
	30 mM	0.1243 mL	0.6214 mL	1.2428 mL	3.1069 mL

Sparstolonin B Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sparstolonin B1259330-61-4Toll-like Receptor (TLR)HIVHuman immunodeficiency virusInhibitorinhibitorinhibit

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Sparstolonin B

Customer Review

Sparstolonin B Related Antibodies

9 Publications Citing Use of MCE Sparstolonin B

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Toll-like Receptor (TLR) Isoform Specific Products:

View All HIV Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Sparstolonin B Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Sparstolonin B Related Classifications

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