2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. C29

C29 

Cat. No.: HY-100461 Purity: ≥98.0%
COA Handling Instructions

C29 is a Toll-like receptor 2 (TLR2) inhibitor. C29 blocks hTLR2/1 and hTLR2/6 signaling with IC50s of 19.7 and 37.6 μM, respectively.

For research use only. We do not sell to patients.

C29 Chemical Structure

C29 Chemical Structure

CAS No. : 363600-92-4

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 188 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 188 In-stock
Solid
1 mg USD 60 In-stock
5 mg USD 130 In-stock
10 mg USD 230 In-stock
50 mg USD 790 In-stock
100 mg USD 1300 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 61 publication(s) in Google Scholar

C29 Related Antibodies

Top Publications Citing Use of Products

58 Publications Citing Use of MCE C29

IF
WB

    C29 purchased from MCE. Usage Cited in: J Control Release. 2020 Dec 10;328:210-221.  [Abstract]

    Western blot analysis show that MyD88 is active in DP7-C-stimulated BMDCs, and the activation of MyD88 expression by DP7-C and DOTAP/DP7-C is inhibited after BMDCs are pretreated with C29.

    C29 purchased from MCE. Usage Cited in: Neurosci Lett. 2018 Nov 1;686:33-40.   [Abstract]

    Images show the expression of MCPIP1 after the treatment of of PBS and LPS (with TAK-242, C29, FPS-ZM1).

    C29 purchased from MCE. Usage Cited in: Neurosci Lett. 2018 Nov 1;686:33-40.   [Abstract]

    Western Blot analyses show the protein expression of MCPIP1 after the treatment of PBS and LPS (with TAK-242, C29, FPS-ZM1).

    View All Toll-like Receptor (TLR) Isoform Specific Products:

    View All Isoforms
    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    C29 is a Toll-like receptor 2 (TLR2) inhibitor. C29 blocks hTLR2/1 and hTLR2/6 signaling with IC50s of 19.7 and 37.6 μM, respectively[1].

    IC50 & Target[1]

    TLR2

     

    In Vitro

    C29 (10 or 50 μΜ; 1 hour) blocks P3C- and P2C-induced IL-8 mRNA dose-dependently in HEK-TLR2 stable transfectants. C29 (50-200 μM; 1 hour) inhibits P3C- and P2C-induced IL-1β gene expression significantly at both 1 h and 4 h following stimulation in THP-1 cells[1].
    ? C29 (25 or 50 μΜ; 1 hour) reduces P3C-induced but not P2C-induced TNF-α mRNA and IL-12 p40 protein significantly in primary murine macrophages[1].
    ? C29 (50 μΜ; 1 hour) blocks TLR2 bacterial agonist-induced proinflammatory gene expression in HEK-TLR2 cells and murine macrophages[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    C29 Related Antibodies

    Western Blot Analysis[1]

    Cell Line: THP-1 cells
    Concentration: 150 μM
    Incubation Time: 1 hours
    Result: Diminished the interaction between endogenous TLR2 and myeloid differentiation primary response gene 88 (MyD88) at 15 and 30 min poststimulation with P3C.

    Western Blot Analysis[1]

    Cell Line: Murine peritoneal macrophages
    Concentration: 50 μM
    Incubation Time: 1 hours
    Result: Blocked robust MAPK activation at 30 min and reduced NF-κB activation from 5 to 30 min.
    Prevented P3C-induced degradation of IκBα at 15 and 30 min.
    Molecular Weight

    285.29

    Appearance

    Solid

    Formula

    C16H15NO4
    CAS No.
    SMILES

    OC1=C(/C=N/C2=C(C)C(C(O)=O)=CC=C2)C=CC=C1OC
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 30 mg/mL (105.16 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5052 mL 17.5260 mL 35.0521 mL
    5 mM 0.7010 mL 3.5052 mL 7.0104 mL
    10 mM 0.3505 mL 1.7526 mL 3.5052 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation
    References
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    C29 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    C29363600-92-4C 29C-29Toll-like Receptor (TLR)Toll-likeTLR2hTLR2/1hTLR2/6Inhibitorinhibitorinhibit

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