1. Immunology/Inflammation
  2. Toll-like Receptor (TLR)
  3. C29

C29 

Cat. No.: HY-100461 Purity: ≥98.0%
Handling Instructions

C29 is a Toll-like receptor 2 (TLR2) inhibitor. C29 blocks hTLR2/1 and hTLR2/6 signaling with IC50s of 19.7 and 37.6 μM, respectively.

For research use only. We do not sell to patients.

C29 Chemical Structure

C29 Chemical Structure

CAS No. : 363600-92-4

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 188 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 188 In-stock
Estimated Time of Arrival: December 31
Solid
1 mg USD 60 In-stock
Estimated Time of Arrival: December 31
5 mg USD 130 In-stock
Estimated Time of Arrival: December 31
10 mg USD 230 In-stock
Estimated Time of Arrival: December 31
50 mg USD 790 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1300 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 34 publication(s) in Google Scholar

Top Publications Citing Use of Products

    C29 purchased from MCE. Usage Cited in: Neurosci Lett. 2018 Nov 1;686:33-40. 

    Western Blot analyses show the protein expression of MCPIP1 after the treatment of PBS and LPS (with TAK-242, C29, FPS-ZM1).

    C29 purchased from MCE. Usage Cited in: Neurosci Lett. 2018 Nov 1;686:33-40. 

    Images show the expression of MCPIP1 after the treatment of of PBS and LPS (with TAK-242, C29, FPS-ZM1).

    C29 purchased from MCE. Usage Cited in: J Control Release. 2020 Dec 10;328:210-221.

    Western blot analysis show that MyD88 is active in DP7-C-stimulated BMDCs, and the activation of MyD88 expression by DP7-C and DOTAP/DP7-C is inhibited after BMDCs are pretreated with C29.

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    Description

    C29 is a Toll-like receptor 2 (TLR2) inhibitor. C29 blocks hTLR2/1 and hTLR2/6 signaling with IC50s of 19.7 and 37.6 μM, respectively[1].

    IC50 & Target[1]

    TLR2

     

    In Vitro

    C29 (10 or 50 μΜ; 1 hour) blocks P3C- and P2C-induced IL-8 mRNA dose-dependently in HEK-TLR2 stable transfectants. C29 (50-200 μM; 1 hour) inhibits P3C- and P2C-induced IL-1β gene expression significantly at both 1 h and 4 h following stimulation in THP-1 cells[1].
    C29 (25 or 50 μΜ; 1 hour) reduces P3C-induced but not P2C-induced TNF-α mRNA and IL-12 p40 protein significantly in primary murine macrophages[1].
    C29 (50 μΜ; 1 hour) blocks TLR2 bacterial agonist-induced proinflammatory gene expression in HEK-TLR2 cells and murine macrophages[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[1]

    Cell Line: THP-1 cells
    Concentration: 150 μM
    Incubation Time: 1 hours
    Result: Diminished the interaction between endogenous TLR2 and myeloid differentiation primary response gene 88 (MyD88) at 15 and 30 min poststimulation with P3C.

    Western Blot Analysis[1]

    Cell Line: Murine peritoneal macrophages
    Concentration: 50 μM
    Incubation Time: 1 hours
    Result: Blocked robust MAPK activation at 30 min and reduced NF-κB activation from 5 to 30 min.
    Prevented P3C-induced degradation of IκBα at 15 and 30 min.
    Molecular Weight

    285.29

    Formula

    C₁₆H₁₅NO₄

    CAS No.
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 30 mg/mL (105.16 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5052 mL 17.5260 mL 35.0521 mL
    5 mM 0.7010 mL 3.5052 mL 7.0104 mL
    10 mM 0.3505 mL 1.7526 mL 3.5052 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    • Molarity Calculator

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    Product Name:
    C29
    Cat. No.:
    HY-100461
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