1. Immunology/Inflammation
  2. Toll-like Receptor (TLR)

C29 

Cat. No.: HY-100461 Purity: >98.0%
Handling Instructions

C29 is a potential TLR2 inhibitor.

For research use only. We do not sell to patients.

C29 Chemical Structure

C29 Chemical Structure

CAS No. : 363600-92-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 188 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
1 mg USD 60 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
5 mg USD 130 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
10 mg USD 230 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
50 mg USD 790 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
100 mg USD 1300 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

C29 is a potential TLR2 inhibitor.

In Vitro

C29 blunts hTLR2/1 and hTLR2/6 signaling in HEK-TLR2 Stable transfectants and THP-1 Cells. C29 blocks P3C- and P2C-induced IL-8 mRNA dose-dependently in HEK-TLR2 stable transfectants. C29 also inhibits P3C- and P2C-induced IL-1β gene expression significantly at both 1 h and 4 h following stimulation, as well as both P3C- and P2C-induced NF-κB–luciferase activity in transiently transfected HEK293T cells expressing hTLR2 and an NF-κB–sensitive luciferase reporter construct. C29 preferentially inhibits TLR2/1 signaling in primary murine macrophages. C29 blocks TLR2 bacterial agonist-induced proinflammatory gene expression in HEK-TLR2 Cells and murine macrophages. C29 inhibits ligand-induced interaction of TLR2 with MyD88 and blocks MAPK and NF-κB activation[1].

In Vivo

C29L inhibits TLR2/1-induced inflammation in mice. Mice treated twice with C29L before administration of P3C significantly blocks IL-12 p40 and TNF-α liver cytokine mRNA and serum protein. C29L has a significant inhibitory effect at the later time point for IL-12 p40[1].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 3.5052 mL 17.5260 mL 35.0521 mL
5 mM 0.7010 mL 3.5052 mL 7.0104 mL
10 mM 0.3505 mL 1.7526 mL 3.5052 mL
Please refer to the solubility information to select the appropriate solvent.
Animal Administration
[1]

Mice: Female C57BL/6J mice receive PBS, water, or C29L (in water) administered i.p. (1.314 mM/g). After 1 h, mice receive a second injection of PBS, water, or C29L administered i.p. (1.314 mM/g) and are subsequently challenged i.p. with PBS or P3C (100 μg) for 1 or 3 h. Mice are bled, and sera are prepared. Livers are also extracted for qRT-PCR analysis[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

285.29

Formula

C₁₆H₁₅NO₄

CAS No.

363600-92-4

SMILES

OC1=C(/C=N/C2=C(C)C(C(O)=O)=CC=C2)C=CC=C1OC

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 30 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
C29
Cat. No.:
HY-100461
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