2. Academic Validation
  3. Perilla Fruit Water Extract Attenuates Inflammatory Responses and Alleviates Neutrophil Recruitment via MAPK/JNK-AP-1/c-Fos Signaling Pathway in ARDS Animal Model

Perilla Fruit Water Extract Attenuates Inflammatory Responses and Alleviates Neutrophil Recruitment via MAPK/JNK-AP-1/c-Fos Signaling Pathway in ARDS Animal Model

  • Evid Based Complement Alternat Med. 2022 Jun 30:2022:4444513. doi: 10.1155/2022/4444513.
Nai-Chun Ting 1,  Yu-Hao Chen 2,  Jeng-Chang Chen 3 4,  Wen-Chung Huang 5 6 7 8,  Chian-Jiun Liou 5 6 7 8,  Li-Chen Chen 7 8,  Sien-Hung Yang 1 6 9 10,  Ming-Ling Kuo 2 6 7 8
Affiliations

Affiliations

  • 1 Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
  • 2 Department of Microbiology and Immunology, Graduate Institute of Biomedical Sciences, Chang Gung University, Taoyuan, Taiwan.
  • 3 Department of Surgery, Chang Gung Memorial Hospital-Linko, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
  • 4 Department of Medicine, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
  • 5 Graduate Institute of Health Industry Technology Research Center for Food and Cosmetic Safety, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
  • 6 Research Center for Chinese Herbal Medicine College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
  • 7 Division of Allergy, Asthma, and Rheumatology Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
  • 8 Department of Pediatrics, New Taipei Municipal Tucheng Hospital, New Taipei, Taiwan.
  • 9 School of Traditional Chinese Medicine, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
  • 10 Division of Chinese Internal Medicine Center for Traditional Chinese Medicine Chang Gung Memorial Hospital, Taoyuan, Taiwan.
PMID: 35815275 DOI: 10.1155/2022/4444513
Abstract

Airway respiratory distress syndrome (ARDS) is usually caused by a severe pulmonary Infection. However, there is currently no effective treatment for ARDS. Traditional Chinese medicine (TCM) has been shown to effectively treat inflammatory lung diseases, but a clear mechanism of action of TCM is not available. Perilla fruit water extract (PFWE) has been used to treat cough, excessive mucus production, and some pulmonary diseases. Thus, we propose that PFWE may be able to reduce lung inflammation and neutrophil infiltration in a lipopolysaccharide (LPS)-stimulated murine model. C57BL/6 mice were stimulated with LPS (10 μg/mouse) by intratracheal (IT) injection and treated with three doses of PFWE (2, 5, and 8 g/kg) by intraperitoneal (IP) injections. To investigate possible mechanisms, A549 cells were treated with PFWE and stimulated with LPS. Our results showed that PFWE decreased airway resistance, neutrophil infiltration, vessel permeability, and interleukin (IL)-6 and chemokine (C-C motif) ligand 2 (CCL2/MCP-1) expressions in vivo. In addition, the PFWE inhibited the expression of IL-6, CCL2/MCP-1, chemokine (CXC motif) ligand 1 (CXCL1/GROα), and IL-8 in vitro. Moreover, PFWE also inhibited the MAPK/JNK-AP-1/c-Fos signaling pathway in A549 cells. In conclusion, we demonstrated that PFWE attenuated pro-inflammatory cytokine and chemokine levels and downregulated neutrophil recruitment through the MAPK/JNK-AP-1/c-Fos pathway. Thus, PFWE can be a potential drug to assist the treatment of ARDS.

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