2. Academic Validation
  3. Hydroxy-β-sanshool isolated from Zanthoxylum piperitum (Japanese pepper) shortens the period of the circadian clock

Hydroxy-β-sanshool isolated from Zanthoxylum piperitum (Japanese pepper) shortens the period of the circadian clock

  • Food Funct. 2022 Sep 22;13(18):9407-9418. doi: 10.1039/d2fo01036d.
Tatsunosuke Tomita 1,  Yasuhiro Kawano 1,  Masahiro Kassai 2,  Hiroyuki Onda 2,  Yoshihiro Nakajima 3,  Koyomi Miyazaki 1
Affiliations

Affiliations

  • 1 Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba 305-8566, Japan. [email protected].
  • 2 S&B Foods Inc., #605 MITSUI LINK-Lab Shinkiba1 Shinkiba 2-3-8, Koto-ku, Tokyo 136-0082, Japan.
  • 3 Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hayashicho 2217-14, Takamatsu, 761-0395, Japan.
PMID: 35960176 DOI: 10.1039/d2fo01036d
Abstract

We showed that an ethanol extract from Zanthoxylum piperitum can shorten the circadian rhythm at the cellular level and that this activity was due to hydroxy-β-sanshool, a secondary metabolite in this plant. An ethanol extract of Z. piperitum was repeatedly fractionated using solid phase extraction and reverse-phase HPLC, then the circadian rhythms of cells to which the fractions were loaded were monitored using real-time reporter gene assays. We purified one HPLC peak and identified it as hydroxy-β-sanshool using liquid chromatography (LC)-precision-mass spectrometry (MS). This compound shortened the period of Bmal1 and Per2 at the cellular level. Incubation cells for 24 h with hydroxy-β-sanshool resulted in upregulated Per2 promoter activity. Hydroxy-β-sanshool also dose-dependently upregulated expression of the clock genes Bmal1, Per1, Per2 and Cry1 and the clock-controlled oxidative stress responsive genes Gpx1and Sod2.

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