Cat. No.: HY-N6825

Hydroxy-α-sanshool

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Hydroxy-α-sanshool is a transient receptor potential ankyrin 1 (TRPA1) and TRP vanilloid 1 (TRPV1) agonist with EC₅₀s of 69 and 1.1 μM, respectively. Hydroxy-α-sanshool can be used for pain research.

For research use only. We do not sell to patients.

Purity: 98.15%
CAS No.: 83883-10-7
Formula: C₁₆H₂₅NO₂
Molecular Weight:263.38

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Hydroxy-α-sanshool Chemical Structure

CAS No.: 83883-10-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

EC50: 69 µM (TRPA1), 1.1 µM (TRPV1)^[1]

All TRP Channel Isoforms :

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	59 nM Compound: 2	Displacement of [3H]-CP55940 from human CB2 receptor expressed in CHO cells preincubated for 1 hr followed by radioligand addition measured after 1 hr by liquid scintillation counting analysis Displacement of [3H]-CP55940 from human CB2 receptor expressed in CHO cells preincubated for 1 hr followed by radioligand addition measured after 1 hr by liquid scintillation counting analysis	[PMID: 24175626]
CHO	IC₅₀	7400 nM Compound: 2	Displacement of [3H]-Win55212-2 from human CB1 receptor expressed in CHO cells preincubated for 1 hr followed by radioligand addition measured after 1 hr by liquid scintillation counting analysis Displacement of [3H]-Win55212-2 from human CB1 receptor expressed in CHO cells preincubated for 1 hr followed by radioligand addition measured after 1 hr by liquid scintillation counting analysis	[PMID: 24175626]

In Vitro

Hydroxy-α-sanshool (0-10 mM; 20 s) induces inward currents in cultured DRG neuron^[2].
Hydroxy-α-sanshool (1 mM; 0-1 min) increases intracellular Ca²⁺ in cultured DRG neuron^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

Dorsal root ganglion (DRG) neurons
Concentration:

0-10 mM
Incubation Time:

20 s
Result:

Induced small but robust inward currents for DRG neuron with an EC₅₀ of 66.2 μM.

Cell Viability Assay^[2]

Cell Line:

Dorsal root ganglion (DRG) neurons
Concentration:

1 mM
Incubation Time:

0-1 min
Result:

Led to an intracellular Ca²⁺ increase in the majority of DRG neurons tested (98 of 100).

In Vivo

Hydroxy-α-sanshool (intradermal injection; 4 mg per mouse; once) shows paw-licking response in wild-type and TRPV1-deficient mice^[2].
Hydroxy-α-sanshool (1 mM) is markedly aversive to WT mice, but in TRPV1 KO mice, the aversion is almost entirely eliminated (P<0.001)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Wild-type and TRPV1-deficient mice^[2]
Dosage:

4 mg per mouse
Administration:

Intradermal injection; 4 mg per mouse; once
Result:

Reduced paw-licking response in TRPV1-deficient mice (160 times, P < 0.05).
Showed paw-licking response in wild-type mice (mean 284.8 times) in 20 min.

Chemical Information

CAS No. 83883-10-7
Appearance Oil
Molecular Weight 263.38
Formula C₁₆H₂₅NO₂
Color White to yellow
SMILES

C/C=C/C=C/C=C\CC/C=C/C(NCC(C)(O)C)=O
Structure Classification
- Alkaloids
- Other Alkaloids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

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Journal Impact Factor

Food Funct

Hydroxy-β-sanshool isolated from Zanthoxylum piperitum (Japanese pepper) shortens the period of the circadian clock. [Abstract]

2022 Sep 22;13(18):9407-9418. PMID: 35960176
Foods

Stability of Hydroxy-α-Sanshool in Medium-Chain Triglyceride Oil and Corresponding Oil/Water Emulsions. [Abstract]

2023 Sep 27;12(19):3589. PMID: 37835243
bioRxiv

Trigeminal innervation and tactile responses in mouse tongue. [Abstract]

2024 May 13:2023.08.17.553449. PMID: 37645855

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (379.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.7968 mL

18.9840 mL

37.9680 mL

5 mM

0.7594 mL

3.7968 mL

7.5936 mL

10 mM

0.3797 mL

1.8984 mL

3.7968 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (9.49 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (9.49 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (7.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Koo JY, et al. Compounds from Sichuan and Melegueta peppers activate, covalently and non-covalently, TRPA1 and TRPV1 channels. Br J Pharmacol. 2009 Aug;157(8):1398-409. [Content Brief]

[2]. Koo JY, et al. Hydroxy-alpha-sanshool activates TRPV1 and TRPA1 in sensory neurons. Eur J Neurosci. 2007 Sep;26(5):1139-47. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.7968 mL	18.9840 mL	37.9680 mL	94.9199 mL
	5 mM	0.7594 mL	3.7968 mL	7.5936 mL	18.9840 mL
	10 mM	0.3797 mL	1.8984 mL	3.7968 mL	9.4920 mL
	15 mM	0.2531 mL	1.2656 mL	2.5312 mL	6.3280 mL
	20 mM	0.1898 mL	0.9492 mL	1.8984 mL	4.7460 mL
	25 mM	0.1519 mL	0.7594 mL	1.5187 mL	3.7968 mL
	30 mM	0.1266 mL	0.6328 mL	1.2656 mL	3.1640 mL
	40 mM	0.0949 mL	0.4746 mL	0.9492 mL	2.3730 mL
	50 mM	0.0759 mL	0.3797 mL	0.7594 mL	1.8984 mL
	60 mM	0.0633 mL	0.3164 mL	0.6328 mL	1.5820 mL
	80 mM	0.0475 mL	0.2373 mL	0.4746 mL	1.1865 mL
	100 mM	0.0380 mL	0.1898 mL	0.3797 mL	0.9492 mL

Keywords

Hydroxy-α-sanshool

3 Cited Publications

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Hydroxy-α-sanshool

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Publications Citing Use of MedChemExpress (MCE) Hydroxy-α-sanshool

All TRP Channel Isoforms

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Hydroxy-α-sanshool Related Classifications

HY-N6825 Related Classifications