2. Academic Validation
  3. Autoradiography on deparaffinized tissue sections - A feasibility study with 68Ga-labeled PET-tracers

Autoradiography on deparaffinized tissue sections - A feasibility study with 68Ga-labeled PET-tracers

  • Appl Radiat Isot. 2022 Nov:189:110425. doi: 10.1016/j.apradiso.2022.110425.
Eva-Maria Klebermass 1,  Anna Dengler 2,  Victoria Weissenböck 2,  Gerda Ricken 3,  Wolfgang Wadsak 4,  Helmut Viernstein 5,  Marcus Hacker 2,  Markus Mitterhauser 6,  Cecile Philippe 7
Affiliations

Affiliations

  • 1 Department of Biomedical Imaging and Image-Guided Therapy, Division of Nuclear Medicine, Medical University of Vienna, 1090, Vienna, Austria; Division of Pharmaceutical Technology and Biopharmaceutics, Department of Pharmaceutical Sciences, University of Vienna, 1090, Vienna, Austria.
  • 2 Department of Biomedical Imaging and Image-Guided Therapy, Division of Nuclear Medicine, Medical University of Vienna, 1090, Vienna, Austria.
  • 3 Division of Neuropathology and Neurochemistry, Department of Neurology, Medical University of Vienna, 1090, Vienna, Austria.
  • 4 Department of Biomedical Imaging and Image-Guided Therapy, Division of Nuclear Medicine, Medical University of Vienna, 1090, Vienna, Austria; CBmed - Center for Biomarker Research in Medicine, 8010, Graz, Austria.
  • 5 Division of Pharmaceutical Technology and Biopharmaceutics, Department of Pharmaceutical Sciences, University of Vienna, 1090, Vienna, Austria.
  • 6 Department of Biomedical Imaging and Image-Guided Therapy, Division of Nuclear Medicine, Medical University of Vienna, 1090, Vienna, Austria; Ludwig Boltzmann Institute Applied Diagnostics, 1090, Vienna, Austria; Institute of Inorganic Chemistry, Faculty of Chemistry, University of Vienna, 1090, Vienna, Austria.
  • 7 Department of Biomedical Imaging and Image-Guided Therapy, Division of Nuclear Medicine, Medical University of Vienna, 1090, Vienna, Austria. Electronic address: [email protected].
PMID: 36030760 DOI: 10.1016/j.apradiso.2022.110425
Abstract

Tissue available for retrospective research questions is often already paraffin-embedded for better preservation. However, in vitro autoradiography (AURA) is normally performed on cryopreserved tissue sections. We hypothesized a) that it would also be feasible with deparaffinized tissue sections, enabling the use of human paraffin-embedded tissue for in vitro AURA and b) that the results would be comparable to those obtained with corresponding cryosections. For that purpose, the clinically relevant oncological targets CXCR4, SSTR and PSMA were evaluated. In vitro AURA on deparaffinized tissue sections was feasible, but only with the two receptor ligands [68Ga]Ga-PentixaFor and [68Ga]Ga-DOTANOC. [68Ga]Ga-PSMA-11 did not show any binding on deparaffinized tissue sections, suggesting that native tissue is required for an interaction between this inhibitor and the enzyme.

Keywords

Autoradiography; Cryo; Gallium-68; In vitro; Paraffin.

Figures
Products