2. Academic Validation
  3. Transcriptome and proteomics conjoint analysis reveal metastasis inhibitory effect of 6-shogaol as ferroptosis activator through the PI3K/AKT pathway in human endometrial carcinoma in vitro and in vivo

Transcriptome and proteomics conjoint analysis reveal metastasis inhibitory effect of 6-shogaol as ferroptosis activator through the PI3K/AKT pathway in human endometrial carcinoma in vitro and in vivo

  • Food Chem Toxicol. 2022 Dec:170:113499. doi: 10.1016/j.fct.2022.113499.
Run-Hui Ma 1,  Zhi-Jing Ni 2,  Kiran Thakur 3,  Carlos L Cespedes-Acuña 4,  Jian-Guo Zhang 5,  Zhao-Jun Wei 6
Affiliations

Affiliations

  • 1 School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
  • 2 School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China; School of Biological Science and Engineering, Collaborative Innovation Center for Food Production and Safety, North Minzu University, Yinchuan, 750021, People's Republic of China.
  • 3 School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China; School of Biological Science and Engineering, Collaborative Innovation Center for Food Production and Safety, North Minzu University, Yinchuan, 750021, People's Republic of China. Electronic address: [email protected].
  • 4 Department of Basic Sciences, University of Bio-Bío, Andrés Bello Avenue, Chillan, Chile. Electronic address: [email protected].
  • 5 School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China; School of Biological Science and Engineering, Collaborative Innovation Center for Food Production and Safety, North Minzu University, Yinchuan, 750021, People's Republic of China. Electronic address: [email protected].
  • 6 School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China; School of Biological Science and Engineering, Collaborative Innovation Center for Food Production and Safety, North Minzu University, Yinchuan, 750021, People's Republic of China. Electronic address: [email protected].
PMID: 36341865 DOI: 10.1016/j.fct.2022.113499
Abstract

Endometrial Cancer remains as one of the widespread female malignancies despite the existing treatment measures mainly surgery, radiotherapy, and chemotherapy. In recent times, studies have focused on medicinal Plants such as ginger due to its multifaceted characteristics compared to conventional medicine. 6-Shogaol is regarded as the main active compound of ginger participating in pharmacological activities and combating various health disorders, especially Cancer. In our study, we compared the effects of 6-gingerol, 6-paradol, and 6-shogaol on Ishikawa cells, and found 6-shogaol as a more effective ingredient against Ishikawa cell proliferation. Moreover, its promoted Ferroptosis, as a result, triggered mitochondrial morphologic alternation, as well as changed iron concentration, GSH and MDA levels. Furthermore, 6-Shogaol inhibited cell metastasis by influencing cell invasion and migration. Finally, 6-shogaol could trigger PI3K/Akt signaling pathways in vitro and in vivo confirmed by western blotting assay and immunohistochemical evaluation. These findings suggest that 6-shogaol can be used as promising functional food component in health diet and in drug target methods for endometrial Cancer therapy.

Keywords

6-Shogaol; Endometrial cancer; Ferroptosis; Ishikawa cell; Metastasis; PI3K/AKT.

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