2. Academic Validation
  3. Melatonin alleviates lead-induced fatty liver in the common carps (Cyprinus carpio) via gut-liver axis

Melatonin alleviates lead-induced fatty liver in the common carps (Cyprinus carpio) via gut-liver axis

  • Environ Pollut. 2022 Nov 22;120730. doi: 10.1016/j.envpol.2022.120730.
Zhiying Miao 1,  Zhiruo Miao 2,  Xiaohua Teng 2,  Shiwen Xu 3
Affiliations

Affiliations

  • 1 College of Life Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
  • 2 College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
  • 3 College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. Electronic address: [email protected].
PMID: 36427828 DOI: 10.1016/j.envpol.2022.120730
Abstract

As a widespread aquatic environmental contaminant, Lead (Pb) can provoke hepatic injury in various Animals. Melatonin (MT) plays a crucial role in the regulation of inflammatory response. Accumulating evidence elucidates exogenous toxins can elicit hepatic lipid metabolic disorders by influencing the gut microbiome. Nevertheless, the effects of Pb on gut microbiota and hepatic lipid metabolism of the common carps, and whether MT can prevent and cure Pb-induced toxicity via regulating microbiome remains unknown. Here, metagenomic and transcriptomic analysis were subsequently implemented to identify the Pb exposure-triggered prominent alternation of gut-liver signal. In the present study the severe intestinal injury and fatty liver formation caused by Pb in common carp were preliminarily determined. Metagenomic analysis confirmed that the gut microbiome dominant phyla, family and genus of the common carps were Fusobacteria, Fusobacteriaceae and Cetobacterium. Meanwhile, lipopolysaccharide (LPS) biosynthesis pathway was regarded as one of the main responsible for Pb exposure. Subsequently, LPS was demonstrated as the Pb-triggered microbial-derived signal of the common carps by ELISA analysis, and involves in the hepatic metabolic disorders via deteriorating the intestinal barrier. Additionally, it confirmed that hepatocytes Ferroptosis associated with Pb-evoked fatty liver of the common carps, and the aggravation of lysosomal dyshomeostasis as well as inhibition of AMPK phosphorylation were referred to lipid metabolic disorders. The results of the present study demonstrated microbial-derived signal induced by aquatic Pb contaminant cause fatty liver formation in the common carps, and the protective effects of MT on Pb toxicity were performed by receding LPS over-synthesis, restraining microbiota-sourced LPS transport, along with attenuation of hepatocytes Ferroptosis.

Keywords

Fatty liver; Ferroptosis; Gut microbiome; Gut-liver axis; Lead; Melatonin.

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