2. Academic Validation
  3. A light-up fluorescence platform based DNA: RNA hybrid G-quadruplet for detecting single nucleotide variant of ctDNA and miRNA-21

A light-up fluorescence platform based DNA: RNA hybrid G-quadruplet for detecting single nucleotide variant of ctDNA and miRNA-21

  • Talanta. 2023 May 15:257:124373. doi: 10.1016/j.talanta.2023.124373.
Gaihua Cao 1,  Keyi Long 1,  Yue Qiu 1,  Lan Peng 2,  Haiyan Han 3,  Yongzhong Wang 4,  Danqun Huo 5,  Changjun Hou 6
Affiliations

Affiliations

  • 1 Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
  • 2 Chongqing Medical and Pharmaceutical College Basic Department, Chongqing, 401331, PR China.
  • 3 Chongqing Institute for Food and Drug Control, Chongqing, 401121, PR China.
  • 4 Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China. Electronic address: [email protected].
  • 5 Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China; Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, 400044, PR China. Electronic address: [email protected].
  • 6 Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China. Electronic address: [email protected].
PMID: 36801760 DOI: 10.1016/j.talanta.2023.124373
Abstract

The nucleic acid assay is an area of great concern in the diagnosis and treatment of breast Cancer. Here, we developed a DNA: RNA hybrid G-quadruplet (HQ) detection platform based on strand displacement amplification (SDA) and Baby Spinach RNA aptamer for single nucleotide variant (SNV) of circulating tumor DNA (ctDNA) and miRNA-21. This was the first in vitro construction of HQ for the biosensor. It found that HQ had much stronger ability to switch on fluorescence of DFHBI-1T than Baby Spinach RNA alone. Taking advantage of the platform and the FspI enzyme with high specificity, the biosensor achieved ultra-sensitive detection of SNV of the ctDNA (PIK3CA H1047R gene) and miRNA-21. The light-up biosensor had high anti-interference ability in complex actual samples. Hence, the label-free biosensor provided a sensitive and accurate method for early diagnosis of breast Cancer. Moreover, it opened a new application model for RNA Aptamers.

Keywords

Circulating tumor DNA (ctDNA); Fluorescence; G-quadruplet; Strand displacement amplification (SDA); miRNA.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-110251
    99.44%, RNA Aptamers Probe