2. Cell Cycle/DNA Damage
  3. DNA Stain
  4. DFHBI-1T

DFHBI-1T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=472 nm/507 nm). DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. DFHBI-1T is used to image RNA in live cells.

For research use only. We do not sell to patients.

DFHBI-1T Chemical Structure

DFHBI-1T Chemical Structure

CAS No. : 1539318-36-9

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Based on 8 publication(s) in Google Scholar

Other Forms of DFHBI-1T:

DFHBI-1T Related Antibodies

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  • Purity & Documentation

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Description

DFHBI-1T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=472 nm/507 nm). DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. DFHBI-1T is used to image RNA in live cells[1][2].

In Vitro

DFHBI-1T (20 μM; for 10 min) increases fluorescence in COS7 cells expressing (CGG)60-Spinach2 over DFHBI (20 μM)[1].
Broccoli-DFHBI-1T has ex/em=472 nm/507 nm and Spinach2-DFHBI-1T has ex/em=482 nm/505 nm[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DFHBI-1T Related Antibodies
Molecular Weight

320.21

Formula

C13H9F5N2O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1N(CC(F)(F)F)C(C)=N/C1=C\C2=CC(F)=C(O)C(F)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (312.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1230 mL 15.6148 mL 31.2295 mL
5 mM 0.6246 mL 3.1230 mL 6.2459 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 5.5 mg/mL (17.18 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 5.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (55.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
Dyeing Example
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1230 mL 15.6148 mL 31.2295 mL 78.0738 mL
5 mM 0.6246 mL 3.1230 mL 6.2459 mL 15.6148 mL
10 mM 0.3123 mL 1.5615 mL 3.1230 mL 7.8074 mL
15 mM 0.2082 mL 1.0410 mL 2.0820 mL 5.2049 mL
20 mM 0.1561 mL 0.7807 mL 1.5615 mL 3.9037 mL
25 mM 0.1249 mL 0.6246 mL 1.2492 mL 3.1230 mL
30 mM 0.1041 mL 0.5205 mL 1.0410 mL 2.6025 mL
40 mM 0.0781 mL 0.3904 mL 0.7807 mL 1.9518 mL
50 mM 0.0625 mL 0.3123 mL 0.6246 mL 1.5615 mL
60 mM 0.0520 mL 0.2602 mL 0.5205 mL 1.3012 mL
80 mM 0.0390 mL 0.1952 mL 0.3904 mL 0.9759 mL
100 mM 0.0312 mL 0.1561 mL 0.3123 mL 0.7807 mL
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DFHBI-1T Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DFHBI-1T1539318-36-9DNA StainRNAaptamersprobeSpinachSpinach2iSpinachBroccolilivecellsInhibitorinhibitorinhibit

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