DFHBI-1T
Based on 18 publication(s) in Google Scholar
DFHBI-1T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=472 nm/507 nm). DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. DFHBI-1T is used to image RNA in live cells.
For research use only. We do not sell to patients.
- Purity: 99.44%
- CAS No.: 1539318-36-9
- Formula: C13H9F5N2O2
- Molecular Weight:320.21
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DFHBI-1T
More- Adv Mater. 2025 Nov 17:e14306. [Abstract]
- Int J Biol Macromol. 2026 Mar:350:151041. [Abstract]
- View. 2026 Mar 3.
- Anal Chem. 2026 Mar 3;98(8):5912-5922. [Abstract]
- Anal Chem. 2024 Jul 9;96(27):10927-10934. [Abstract]
- Anal Chem. 2023 Sep 19;95(37):13762-13768. [Abstract]
- Talanta. 2024 Mar 1:269:125465. [Abstract]
- Talanta. 2023 May 15:257:124373. [Abstract]
- RSC Adv. 2024 Mar 26;14(15):10146-10151. [Abstract]
- Anal Chim Acta. 2022 May 29;1209:339816. [Abstract]
- Microchem J. 2025 Oct 21;219:115817.
- Biosci Rep. 2021 Sep 30;41(9):BSR20211525. [Abstract]
- Microbiol Spectr. 2023 Feb 14;11(1):e0323922. [Abstract]
- J Fluoresc. 2026 Jun;36(6):3899-3908. [Abstract]
- J Cell Biochem. 2024 Dec;125(12):e30249. [Abstract]
- Res Sq. 2025 Nov 4:rs.3.rs-7958216. [Abstract]
- Res Sq. 2025 Jun 19.
- SSRN. 2025 Jun 3.
Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
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Cell Imaging/Staining
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Flow Cytometry
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Preparation of stock solution:
Use anhydrous DMSO to prepare a 50 mM DFHBI-1T stock solution.
2. Preparation of working solution:
Dilute the stock solution with serum-free medium to prepare a 20 μM DFHBI-1T working solution.
Note: Please adjust the concentration of DFHBI-1T working solution according to actual conditions and prepare it before use.
3. Specific staining steps:
1) Inoculate cells expressing Spinach2-tagged RNA in a suitable culture dish or culture plate and culture under standard cell culture conditions.
2) Aspirate the old medium and gently wash the cells twice with pre-warmed PBS to remove residual serum and cell debris.
3) Add 20 μM DFHBI-1T working solution to the cells, ensuring complete coverage of the cell monolayer.
4) Incubate the cells with the dye for 10 min in a 37°C, 5% CO2 humidified incubator.
5) After incubation, aspirate the dye-containing medium and wash the cells three times with pre-warmed PBS to remove unbound DFHBI-1T.
6) Replace PBS with fresh cell culture medium or imaging buffer.
7) Immediately image the cells using a fluorescence microscope equipped with a GFP filter set to detect fluorescence of the Spinach2-DFHBI-1T complex.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1539318-36-9
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Appearance Solid
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Molecular Weight 320.21
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Formula C13H9F5N2O2
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Color Light yellow to yellow
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SMILES
O=C1N(CC(F)(F)F)C(C)=N/C1=C\C2=CC(F)=C(O)C(F)=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (18)
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Journal Impact Factor
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Most Recent
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Adv Mater
2025 Nov 17:e14306. PMID: 41243624 -
Int J Biol Macromol
Structure-guided engineering of modular RNA aptamer biosensors for one-pot, multiplex detection of CircRNAs. [Abstract]2026 Mar:350:151041. PMID: 41730444 -
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Anal Chem
An Easy-To-Use and Field-Deployable Cell-Free Bioreporter for Environmental Detection of Tetracyclines and Macrolides. [Abstract]2026 Mar 3;98(8):5912-5922. PMID: 41693084 -
Anal Chem
Label-Free and DNAzyme-Mediated Biosensor with a High Signal-to-Noise Ratio for a Lumpy Skin Disease Virus Assay. [Abstract]2024 Jul 9;96(27):10927-10934. PMID: 38934225 -
Anal Chem
Live-Cell Imaging of Endogenous RNA with a Genetically Encoded Fluorogenic Allosteric Aptamer. [Abstract]2023 Sep 19;95(37):13762-13768. PMID: 37661353 -
Talanta
A binary system based DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. [Abstract]2024 Mar 1:269:125465. PMID: 38008022 -
Talanta
A light-up fluorescence platform based DNA: RNA hybrid G-quadruplet for detecting single nucleotide variant of ctDNA and miRNA-21. [Abstract]2023 May 15:257:124373. PMID: 36801760
DFHBI-1T purchased from MedChemExpress. Usage Cited in: Talanta. 2023 May 15:257:124373. [Abstract]
DFHBI-1T (12.5 mM; 3 min). The feasibility of the SDAHQ platform.
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RSC Adv
In situ imaging of mRNA transcripts based on split-aptamer and split protein in living cells. [Abstract]2024 Mar 26;14(15):10146-10151. PMID: 38544938 -
Anal Chim Acta
2022 May 29;1209:339816. PMID: 35569846
DFHBI-1T purchased from MedChemExpress. Usage Cited in: Anal Chim Acta. 2022 May 29;1209:339816. [Abstract]
Normalized fluorescence intensity of a single 20 μM fluorophore (DFHBI or DFHBI-1T) and in complex with Spinach, Broccoli, and dBroccoli in HeLa cells. Count the median of different group flow cytometry green fluorescence intensity data and use dBroccoli/DFHBI-1T as 1 to calculate the normalized fluorescence intensity. Error bars indicate standard deviations (n = 3).
DFHBI-1T purchased from MedChemExpress. Usage Cited in: Anal Chim Acta. 2022 May 29;1209:339816. [Abstract]
Confocal images of control (untreated), only DFHBI-1T (20 μM), dBroccoli/DFHBI-1T, and UTRsSOD2-dBroccoli/DFHBI-1T in HeLa cells.
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Biosci Rep
Combining protein and RNA quantification to evaluate promoter activity by using dual-color fluorescent reporting systems. [Abstract]2021 Sep 30;41(9):BSR20211525. PMID: 34467968
DFHBI-1T purchased from MedChemExpress. Usage Cited in: Biosci Rep. 2021 Sep 30;41(9):BSR20211525. [Abstract]
DFHBI-1T could be bonded and activated by Broccoli to produce green fluorescence. DFHBI-1T was treated at 20 μM for 10 min at room temperature. The fluorescence imaging of HeLa cell after 24 h transfection. Green signals: Broccoli, Red signals: mCherry.
DFHBI-1T purchased from MedChemExpress. Usage Cited in: Biosci Rep. 2021 Sep 30;41(9):BSR20211525. [Abstract]
DFHBI-1T could be bonded and activated by Broccoli to produce green fluorescence. DFHBI-1T was treated at 20 μM for 10 min at room temperature. Flow cytometry histograms showing the green fluorescence intensity of Broccoli induced by different promoters.
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Microbiol Spectr
Pseudomonas aeruginosa Citrate Synthase GltA Influences Antibiotic Tolerance and the Type III Secretion System through the Stringent Response. [Abstract]2023 Feb 14;11(1):e0323922. PMID: 36602339 -
J Fluoresc
Label-Free Fluorescent Detection of T4 PNK Using a DNA Mimic Green Fluorescent Protein-Based Turn-On Biosensor. [Abstract]2026 Jun;36(6):3899-3908. PMID: 42209914 -
J Cell Biochem
Trans-cleaving hammerhead ribozyme in specific regions can improve knockdown efficiency in vivo. [Abstract]2024 Dec;125(12):e30249. PMID: 35411616 -
Res Sq
2025 Nov 4:rs.3.rs-7958216. PMID: 41282238 -
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Solvent & Solubility
DMSO : 100 mg/mL (312.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Wenjiao Song, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136(4):1198-201. [Content Brief]
[2]. Grigory S Filonov, et al. Broccoli: rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution. J Am Chem Soc. 2014 Nov 19;136(46):16299-308. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1230 mL | 15.6148 mL | 31.2295 mL | 78.0738 mL |
| 5 mM | 0.6246 mL | 3.1230 mL | 6.2459 mL | 15.6148 mL | |
| 10 mM | 0.3123 mL | 1.5615 mL | 3.1230 mL | 7.8074 mL | |
| 15 mM | 0.2082 mL | 1.0410 mL | 2.0820 mL | 5.2049 mL | |
| 20 mM | 0.1561 mL | 0.7807 mL | 1.5615 mL | 3.9037 mL | |
| 25 mM | 0.1249 mL | 0.6246 mL | 1.2492 mL | 3.1230 mL | |
| 30 mM | 0.1041 mL | 0.5205 mL | 1.0410 mL | 2.6025 mL | |
| 40 mM | 0.0781 mL | 0.3904 mL | 0.7807 mL | 1.9518 mL | |
| 50 mM | 0.0625 mL | 0.3123 mL | 0.6246 mL | 1.5615 mL | |
| 60 mM | 0.0520 mL | 0.2602 mL | 0.5205 mL | 1.3012 mL | |
| 80 mM | 0.0390 mL | 0.1952 mL | 0.3904 mL | 0.9759 mL | |
| 100 mM | 0.0312 mL | 0.1561 mL | 0.3123 mL | 0.7807 mL |
DFHBI-1T Related Classifications
HY-110251 Related Classifications
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