2. Cell Cycle/DNA Damage
  3. DNA Stain
  4. DFHBI-1T

DFHBI-1T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=472 nm/507 nm). DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. DFHBI-1T is used to image RNA in live cells.

For research use only. We do not sell to patients.

CAS No. : 1539318-36-9

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Customer Review

Based on 17 publication(s) in Google Scholar

Other Forms of DFHBI-1T:

DFHBI-1T Related Antibodies

Top Publications Citing Use of Products

    DFHBI-1T purchased from MedChemExpress. Usage Cited in: Talanta. 2023 May 15:257:124373.

    DFHBI-1T (12.5 mM). The feasibility of the SDAHQ platform.

    DFHBI-1T purchased from MedChemExpress. Usage Cited in: Anal Chim Acta. 2022 May 29;1209:339816.  [Abstract]

    Normalized fluorescence intensity of a single 20 μM fluorophore (DFHBI or DFHBI-1T) and in complex with Spinach, Broccoli, and dBroccoli in HeLa cells. Count the median of different group flow cytometry green fluorescence intensity data and use dBroccoli/DFHBI-1T as 1 to calculate the normalized fluorescence intensity. Error bars indicate standard deviations (n = 3).

    DFHBI-1T purchased from MedChemExpress. Usage Cited in: Anal Chim Acta. 2022 May 29;1209:339816.  [Abstract]

    Confocal images of control (untreated), only DFHBI-1T (20 μM), dBroccoli/DFHBI-1T, and UTRsSOD2-dBroccoli/DFHBI-1T in HeLa cells.

    DFHBI-1T purchased from MedChemExpress. Usage Cited in: Biosci Rep. 2021 Sep 30;41(9):BSR20211525.  [Abstract]

    DFHBI-1T could be bonded and activated by Broccoli to produce green fluorescence. DFHBI-1T was treated at 20 μM for 10 min at room temperature. The fluorescence imaging of HeLa cell after 24 h transfection. Green signals: Broccoli, Red signals: mCherry.

    DFHBI-1T purchased from MedChemExpress. Usage Cited in: Biosci Rep. 2021 Sep 30;41(9):BSR20211525.  [Abstract]

    DFHBI-1T could be bonded and activated by Broccoli to produce green fluorescence. DFHBI-1T was treated at 20 μM for 10 min at room temperature. Flow cytometry histograms showing the green fluorescence intensity of Broccoli induced by different promoters.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DFHBI-1T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=472 nm/507 nm). DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. DFHBI-1T is used to image RNA in live cells[1][2].

    In Vitro

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
    1. Preparation of stock solution:
    Use anhydrous DMSO to prepare a 50 mM DFHBI-1T stock solution.
    2. Preparation of working solution:
    Dilute the stock solution with serum-free medium to prepare a 20 μM DFHBI-1T working solution.
    Note: Please adjust the concentration of DFHBI-1T working solution according to actual conditions and prepare it before use.
    3. Specific staining steps:
    1) Inoculate cells expressing Spinach2-tagged RNA in a suitable culture dish or culture plate and culture under standard cell culture conditions. 2) Aspirate the old medium and gently wash the cells twice with pre-warmed PBS to remove residual serum and cell debris.
    3) Add 20 μM DFHBI-1T working solution to the cells, ensuring complete coverage of the cell monolayer.
    4) Incubate the cells with the dye for 10 min in a 37°C, 5% CO2 humidified incubator.
    5) After incubation, aspirate the dye-containing medium and wash the cells three times with pre-warmed PBS to remove unbound DFHBI-1T.
    6) Replace PBS with fresh cell culture medium or imaging buffer.
    7) Immediately image the cells using a fluorescence microscope equipped with a GFP filter set to detect fluorescence of the Spinach2-DFHBI-1T complex.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DFHBI-1T Related Antibodies
    Molecular Weight

    320.21

    Formula

    C13H9F5N2O2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1N(CC(F)(F)F)C(C)=N/C1=C\C2=CC(F)=C(O)C(F)=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (312.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1230 mL 15.6148 mL 31.2295 mL
    5 mM 0.6246 mL 3.1230 mL 6.2459 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation
    Dyeing Example
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.1230 mL 15.6148 mL 31.2295 mL 78.0738 mL
    5 mM 0.6246 mL 3.1230 mL 6.2459 mL 15.6148 mL
    10 mM 0.3123 mL 1.5615 mL 3.1230 mL 7.8074 mL
    15 mM 0.2082 mL 1.0410 mL 2.0820 mL 5.2049 mL
    20 mM 0.1561 mL 0.7807 mL 1.5615 mL 3.9037 mL
    25 mM 0.1249 mL 0.6246 mL 1.2492 mL 3.1230 mL
    30 mM 0.1041 mL 0.5205 mL 1.0410 mL 2.6025 mL
    40 mM 0.0781 mL 0.3904 mL 0.7807 mL 1.9518 mL
    50 mM 0.0625 mL 0.3123 mL 0.6246 mL 1.5615 mL
    60 mM 0.0520 mL 0.2602 mL 0.5205 mL 1.3012 mL
    80 mM 0.0390 mL 0.1952 mL 0.3904 mL 0.9759 mL
    100 mM 0.0312 mL 0.1561 mL 0.3123 mL 0.7807 mL
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    DFHBI-1T Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DFHBI-1T1539318-36-9DNA StainRNAaptamersprobeSpinachSpinach2iSpinachBroccolilivecellsInhibitorinhibitorinhibit

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