Light-Up Nanostructures with Allosterically Controlled Fluorogenic DNA Aptamers
- Adv Mater. 2025 Nov 17:e14306. doi: 10.1002/adma.202514306.
Affiliations
- 1. Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou, 310022, China.
- 2. Tsinghua-Peking Joint Center for Life Sciences, Beijing, 100084, China.
- 3. School of Basic Medical Sciences and Institute for Immunology, Tsinghua University, Beijing, 100084, China.
- 4. Beijing Key Laboratory of Immunological Research of Allergy, Beijing, 100084, China.
- 5. School of Life Sciences, Tsinghua University, Beijing, 100084, China.
- 6. Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China.
- 7. Beijng Key Laboratory of Biomolecular Data Storage, BGI Research, Beijing, 102601, China.
- 8. State Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen, 518083, China.
- 9. BGI Research, Changzhou, 213299, China.
- 10. Department of Biomedical Engineering, Chinese University of Hong Kong, Hong Kong, 999077, China.
PMID: 41243624
DOI: 10.1002/adma.202514306
Abstract
In this study, a systematic approach is presented for the rational design of synthetic allosteric DNA Aptamers. This methodology enables precise control over the allosteric ON-OFF transition in fluorescent DNA Aptamers, allowing for the engineering of Aptamers with highly tunable fluorescent properties. When combined with toehold-mediated strand displacement, a series of allosteric Aptamers is developed in which the target sequence functions as a specific allosteric modulator. Furthermore, these Aptamers are applied in synthetic DNA computing and in the construction of responsive nanostructures that light up upon activation.
Keywords
DNA computing; DNA nanotechnology; allosteric regulation; fluorogenic DNA aptamer.