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  3. Investigation on the immunotoxicity induced by Emamectin benzoate on THP-1 macrophages based on metabolomics analysis

Investigation on the immunotoxicity induced by Emamectin benzoate on THP-1 macrophages based on metabolomics analysis

  • Environ Toxicol. 2023 Mar 9. doi: 10.1002/tox.23747.
Ziyi Wei 1,  Weiguo Wang 1,  Hao Feng 1,  Wenping Xu 1,  Liming Tao 1,  Zhong Li 1 2,  Yang Zhang 1,  Xusheng Shao 1 2 3 4
Affiliations

Affiliations

  • 1 Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
  • 2 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
  • 3 Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
  • 4 Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
PMID: 36896474 DOI: 10.1002/tox.23747
Abstract

Emamectin benzoate (EMB) is an insecticide extensively used in agricultural area. Assessing the toxic effects of EMB in mammals or humans and its endogenous metabolites alteration are the appropriate means of evaluating its risks to human health. In the study, THP-1 macrophage, a human immune model, was applied to investigate the immunotoxicity of EMB. A global metabolomics approach was developed to analyze metabolic perturbation on macrophages and discover the potential biomarkers of EMB-induced immunotoxicity. The results indicated that EMB could inhibit immune functions of macrophages. Based on metabolomics analysis, our results illustrated that EMB caused significant alterations in metabolic profiles on macrophages. 22 biomarkers associated with immune response were screened by pattern recognition and multivariate statistical analysis. Furthermore, pathway analysis identified purine metabolism was the most relevant pathway in the metabolic process and the abnormal conversion of AMP to xanthosine regulated by NT5E might be a potential mechanism of immunotoxicity induced by EMB. Our study provides important insights for understanding and underlying mechanism of immunotoxicity exposed to EMB.

Keywords

Emamectin benzoate; THP-1 macrophages; immunotoxicity; metabolomics; pesticide; purine metabolism.

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