2. Academic Validation
  3. Molecular docking and anti-ulcerative potential of Cucumis (L. Inodorous) on ibuprofen induced gastric ulceration in male wistar animals

Molecular docking and anti-ulcerative potential of Cucumis (L. Inodorous) on ibuprofen induced gastric ulceration in male wistar animals

  • Biomed Pharmacother. 2023 May:161:114531. doi: 10.1016/j.biopha.2023.114531.
Grace Adebayo-Gege 1,  Zainab Shehu Uthman 2,  Moses Dele Adams 3,  Tarfa Florence 4,  Danazumi Umar Haruna 5,  Ngabea Murtala Audu 6,  Hamidu Jabba Lawan 2,  Ozegbe Queen 2,  Onwuchekwa Chinedu 7,  Ajibola Meraiyebu 2,  Ojo Kafaru 2
Affiliations

Affiliations

  • 1 Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine and Health Sciences, Baze University, Abuja, Nigeria. Electronic address: [email protected].
  • 2 Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine and Health Sciences, Baze University, Abuja, Nigeria.
  • 3 Clinical Biochemistry, Phyto-pharmacology and Biochemical Toxicology Research Unit, Department of Biochemistry, Baze University, Abuja, Nigeria. Electronic address: [email protected].
  • 4 Department of Medicinal Chemistry and Quality Control, National Institute for Pharmaceutical Research and Development, Idu-Abuja, Nigeria.
  • 5 Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medicine and Health Sciences, Baze University, Abuja, Nigeria.
  • 6 Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine and Health Sciences, Baze University, Abuja, Nigeria; Department of Medicine, Maitama District Hospital, Abuja, Nigeria.
  • 7 Department of Physiology, Faculty of Basic Medical Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.
PMID: 36934555 DOI: 10.1016/j.biopha.2023.114531
Abstract

Background: The use of NSAIDs have caused stomach injury by inhibiting endogenous mucosal prostaglandin production. Cucumis melo is reported to possess antiulcer potential. This study investigates the mechanism underlying the antiulcer potentials of Cucumis melo (CUM).

Methods: Thirty-five male Wistar rat were randomly assigned to each of seven groups; A(control given water and rat pellets), B(gastric ulcer induced with ibuprofen 400 mg/kg), C (Misoprotol 200 μg/kg), D to G (pretreated with different variation of CUM extract; 25 %, 50 %, 75 % and 100 % at a dose of 1 ml/kg for 3 weeks prior to gastric ulcer induction). Ulcer score, ulcer index and percentage inhibition, total gastric acidity was measured. Antioxidant activities, Malondialdehyde, H+/K+ ATPase, PGE2, TNF-α was done by spectrophotometry. Molecular docking investigation of Cucumis melo compounds against Prostaglandin E2 was carried out. Level of significance was tested at P ≤ 0.05 using Tukey post hoc.

Result: Total gastric acidity, ulcer score, ulcer index, MDA, TNF-α significantly decreased after CUM treatment when compared to group B. The percentage inhibition, antioxidant activities, PGE2 concentration was significantly increased in all treatment groups compared to group B. Interactions of selected compounds of CUM with Prostaglandin E2 at various docking pockets showed folic acid has highest binding affinity followed by delta7-avenasterol and codisterol to PGE2 receptor. this study shows that one of the mechanisms by which CUM exhibits its antiulcer potential by enhancing Prostaglandin synthesis and antioxidant capacity. Therefore, Cucumis melo can therefore be explored as novel antiulcer agents.

Keywords

Cucumis melo; Delta7-avenasterol; Folic acid; Gastric ulcer; Ibuprofen; Prostaglandin E2 receptor.

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