1. Academic Validation
  2. AC010883.5 promotes cell proliferation, invasion, migration, and epithelial-to-mesenchymal transition in cervical cancer by modulating the MAPK signaling pathway

AC010883.5 promotes cell proliferation, invasion, migration, and epithelial-to-mesenchymal transition in cervical cancer by modulating the MAPK signaling pathway

  • BMC Cancer. 2023 Apr 21;23(1):364. doi: 10.1186/s12885-023-10825-2.
Qiyu Gan # 1 Xia Huang # 2 Wenrong Zhao # 1 Hui Liu 1 Yan Xu 3 Xiaohua Zhang 2 Jingxin Cheng 4 5 Rui Chen 6
Affiliations

Affiliations

  • 1 Department of Gynecology and Obstetrics, School of Medicine, Shanghai East Hospital, Tongji University, Shanghai, 200120, China.
  • 2 Department of Gynecology and Obstetrics, Shanghai East Hospital Ji'an Hospital, 80 Ji'an South Road, Ji'an City, 343000, Jiangxi, China.
  • 3 Department of Pathology, School of Medicine, Shanghai East Hospital, Tongji University, Shanghai, 200120, China.
  • 4 Department of Gynecology and Obstetrics, School of Medicine, Shanghai East Hospital, Tongji University, Shanghai, 200120, China. [email protected].
  • 5 Department of Gynecology and Obstetrics, Shanghai East Hospital Ji'an Hospital, 80 Ji'an South Road, Ji'an City, 343000, Jiangxi, China. [email protected].
  • 6 Department of Gynecology, United Family Hospital, Shanghai, China. [email protected].
  • # Contributed equally.
Abstract

Homo sapiens chromosome 2 clone RP11-339H12 (AC010883.5) is a dysregulated long non-coding RNA (lncRNA) that has never been investigated in cervical Cancer (CC). Thus, the potential function and molecular mechanism remain unclear. Our study explored the biological function of AC010883.5 to determine the underlying mechanisms in CC and provide potential therapeutic targets for improving the clinical treatment strategy. We used quantitative real-time polymerase chain reaction to measure mitochondrial RNA levels and western blot to measure the protein levels of target genes. Further, we used Cell Counting Kit-8 and 5-Bromo-2'-deoxyuridine incorporation assays to evaluate cell proliferation in vitro. Cell Apoptosis was analyzed by flow cytometry. Cell invasion was analyzed by wound healing and Transwell migration assays was ued to analyze cell migration. Finally, the biological function and mechanism of AC010883.5 in CC growth were evaluated by in vivo xenograft assay. AC010883.5 was enhanced in CC tissues and cell lines, and enhanced AC010883.5 expression accelerated CC cell proliferation, migration, and invasion and induced epithelial-mesenchymal transition in vitro and in vivo. AC010883.5 also activated the mitogen-activated protein kinase (MAPK) signaling pathway by promoting phosphorylation of extracellular signal-regulated kinase 1/2 (i.e., ERK1/2) and MAPK kinase 1/2 (i.e., MEK1/2). Blocking the MAPK signaling pathway could counteract the pro-proliferative, pro-migrative, and pro-invasive effects of AC010883.5 over-expression. We found that the lncRNA, AC010883.5, is an oncogenic molecule involved in CC tumor progression via dysregulation of the MAPK signaling pathway, implying that AC010883.5 could be a tumor progression and therapeutic response biomarker.

Keywords

AC010883.5; Cervical cancer; ERK1/2; MAPK signaling pathway; MEK1/2.

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