2. Academic Validation
  3. Flubendiamide induced genetic and cellular damages directly influence the life cycle of the oriental leaf worm, Spodoptera litura

Flubendiamide induced genetic and cellular damages directly influence the life cycle of the oriental leaf worm, Spodoptera litura

  • Pestic Biochem Physiol. 2023 Jun:193:105448. doi: 10.1016/j.pestbp.2023.105448.
Mohd Jameel 1,  Md Fazle Alam 2,  Homa Fatma 1,  Deepti Singh 1,  Mohammad Afsar Khan 1,  Mohd Aamir Qureshi 3,  Saleem Javed 3,  Hina Younus 4,  Khowaja Jamal 5,  Hifzur R Siddique 6
Affiliations

Affiliations

  • 1 Department of Zoology, Aligarh Muslim University, Aligarh 202002, India.
  • 2 Institute of Biomedical Science, Fudan University, Shanghai 200437, China; Department of Biomedical Sciences, College of Rockford, University of Illinois, Chicago, United States of America.
  • 3 Department of Biochemistry, Aligarh Muslim University, Aligarh 202002, India.
  • 4 Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
  • 5 Department of Zoology, Aligarh Muslim University, Aligarh 202002, India. Electronic address: [email protected].
  • 6 Department of Zoology, Aligarh Muslim University, Aligarh 202002, India. Electronic address: [email protected].
PMID: 37248017 DOI: 10.1016/j.pestbp.2023.105448
Abstract

Indiscriminate uses of Insecticide greatly damage the environment as well as non-target organisms. Thus, multiple levels of bioassays can help better management of our environment. Flubendiamide is a phthalic acid diamide Insecticide that ceases the function of insect muscle leading to paralysis and death. Here, we aimed to explore the effects of Flubendiamide on the life cycle of Spodoptera litura vis-a-vis the mode of action. Fourth instar larvae of the same age (120 ± 2 h) and size were fed with different concentrations (20-80 μg/mL) of Flubendiamide for 12-72 h. We performed a pharmacokinetics study, different biochemical assays, p450, Ecdysone receptor (EcR) and Other genes expression analyses by Real-Time PCR and gross damages by Dye exclusion assay and histopathology. Our results demonstrate that the mean concentration of Flubendiamide after 48 h is 9.907 μg/mL and (i) altered the molting, metamorphosis, and reproduction at 80 μg/mL (24 h) (ii) increases all oxidative stress parameters (ROS/RNS, MDA, 8OHdG), decreases oxidative defense mechanisms (SOD, CAT, GST) at 80 μg/mL (48 h) and p450 in a time and concentration-dependent manner, (iii) activates CncC/Maf apoptotic pathways at 80 μg/mL concentration at 24 h while the expression declined from 48 h onwards, (iii) downregulates the EcR expression in a time and concentration-dependent manner, which might be responsible for disturbed molting, metamorphosis, and reproduction, and (iv) increase the expression of apoptotic genes (Caspase 1, -3, and - 5), in time and concentration-dependent manner causing gross morphological and histological damages. In conclusion, indiscriminate use of this Insecticide can affect the ecosystem and have the capacity to cause multiple hazardous effects on experimental organisms. Thus, it warrants further investigations to improve and optimize the integrated pest management packages, including Flubendiamide for better management.

Keywords

Apoptosis; CncC/Maf signaling axis; Ecdysone receptor; Flubendiamide; Insecticide; Life cycle; Oxidative stress.

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