2. Academic Validation
  3. Paeoniflorin found in Paeonia lactiflora root extract inhibits melanogenesis by regulating melanin-related signal transduction in B16F10 cells

Paeoniflorin found in Paeonia lactiflora root extract inhibits melanogenesis by regulating melanin-related signal transduction in B16F10 cells

  • J Cosmet Dermatol. 2023 Jun 8. doi: 10.1111/jocd.15789.
Su-Ying Wen 1 2,  Ya-Shian Wu 3 4,  Hsun Liu 3 4,  Shang-Chuan Ng 3 4,  Viswanadha Vijaya Padma 5,  Chih-Yang Huang 6 7 8 9 10,  Wei-Wen Kuo 3 4
Affiliations

Affiliations

  • 1 Department of Dermatology, Taipei City Hospital, Taipei, Taiwan,ROC.
  • 2 Center for General Education, Mackay Junior College of Medicine, Nursing, and Management, Taipei, Taiwan, ROC.
  • 3 Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung, Taiwan, ROC.
  • 4 Ph.D. Program for Biotechnology Industry, China Medical University, Taichung, Taiwan, ROC.
  • 5 Translational Research Laboratory, Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore, India.
  • 6 Cardiovascular and Mitochondrial Related Disease Research Center, Buddhist Tzu Chi Medical Foundation, Hualien Tzu Chi Hospital, Hualien, Taiwan, ROC.
  • 7 Center of General Education, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology, Hualien, Taiwan, ROC.
  • 8 Department of Medical Research, China Medical University Hospital, China Medical University, Taiwan, ROC.
  • 9 Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, ROC.
  • 10 Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung, Taiwan, ROC.
PMID: 37288793 DOI: 10.1111/jocd.15789
Abstract

Background: Skin pigmentation is modulated by various processes, with melanogenesis playing a key role. Melanin is synthesized by the catalysis of melanogenesis-related enzymes, such as Tyrosinase and tyrosine-related proteins TRP-1 and TRP-2. Paeoniflorin is the main bioactive component of Paeonia suffruticosa Andr., Paeonia lactiflora., or Paeonia veitchii Lynch and has been used for centuries for its anti-inflammatory, anti-oxidant, and anti-carcinogenic properties.

Aims & methods: In this study, melanin biosynthesis in mouse melanoma (B16F10) cells was induced using α-melanocyte-stimulating hormone (α-MSH), and then cells were co-treated with paeoniflorin to evaluate its potential anti-melanogenic effect.

Results: α-MSH stimulation increased melanin content, Tyrosinase activity, and melanogenesis-related markers in a dose-dependent manner. However, treatment with paeoniflorin reversed α-MSH-induced upregulation of melanin content and Tyrosinase activity. Furthermore, paeoniflorin inhibited cAMP response element-binding protein activation and TRP-1, TRP-2, and microphthalmia-associated transcription factor protein expression in α-MSH-stimulated B16F10 cells.

Conclusion: Overall, these findings show the potential of paeoniflorin as a depigmenting agent for cosmetic products.

Keywords

hyperpigmentation; melanoma; paeoniflorin; skin pigmentation; whitening agent.

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