2. Academic Validation
  3. Cryo-XPS for Surface Characterization of Nanomedicines

Cryo-XPS for Surface Characterization of Nanomedicines

  • J Phys Chem A. 2023 Sep 21. doi: 10.1021/acs.jpca.3c03879.
David J H Cant 1,  Yiwen Pei 1,  Andrey Shchukarev 2,  Madeleine Ramstedt 2,  Sara S Marques 3,  Marcela A Segundo 3,  Jeremie Parot 4,  Alicja Molska 4,  Sven E Borgos 4,  Alexander G Shard 1,  Caterina Minelli 1
Affiliations

Affiliations

  • 1 National Physical Laboratory, Hampton Road, Teddington TW11 0LW, U.K.
  • 2 Department of Chemistry, Umeå University, 901 87 Umeå, Sweden.
  • 3 LAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
  • 4 Department of Biotechnology and Nanomedicine, SINTEF Industry, 7465 Trondheim, Norway.
PMID: 37733882 DOI: 10.1021/acs.jpca.3c03879
Abstract

Nanoparticles used for medical applications commonly possess coatings or surface functionalities intended to provide specific behavior in vivo, for example, the use of PEG to provide stealth properties. Direct, quantitative measurement of the surface chemistry and composition of such systems in a hydrated environment has thus far not been demonstrated, yet such measurements are of great importance for the development of nanomedicine systems. Here we demonstrate the first use of cryo-XPS for the measurement of two PEG-functionalized nanomedicines: a polymeric drug delivery system and a lipid nanoparticle mRNA carrier. The observed differences between cryo-XPS and standard XPS measurements indicate the potential of cryo-XPS for providing quantitative measurements of such nanoparticle systems in hydrated conditions.

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