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  3. A binary system based DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells

A binary system based DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells

  • Talanta. 2024 Mar 1:269:125465. doi: 10.1016/j.talanta.2023.125465.
Tong Li 1,  Mengxu Sun 1,  Suping Xia 1,  Ting Huang 1,  Rong-Tian Li 2,  Chunrong Li 3,  Zong Dai 4,  Jin-Xiang Chen 5,  Jun Chen 6,  Nuan Jia 7
Affiliations

Affiliations

  • 1 NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, PR China.
  • 2 Southern University of Science and Technology Hospital, Shenzhen, 518055, PR China.
  • 3 Qiannan Medical College for Nationalities, Duyun, 558000, PR China.
  • 4 Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen, 518107, PR China.
  • 5 NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, PR China. Electronic address: [email protected].
  • 6 NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, PR China; Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Qingdao University of Science and Technology, Qingdao, 266042, PR China. Electronic address: [email protected].
  • 7 Southern University of Science and Technology Hospital, Shenzhen, 518055, PR China. Electronic address: [email protected].
PMID: 38008022 DOI: 10.1016/j.talanta.2023.125465
Abstract

Developing simple, rapid and specific mRNA imaging strategy plays an important role in the early diagnosis of Cancer and the new drugs development. Herein, we have established a novel binary system based DNA tetrahedron and fluorogenic RNA Aptamers for highly specific and label-free mRNA imaging in living cells. This developed system consisted of tetrahedron probe A (TPA) and tetrahedron probe B (TPB). TK1 mRNA was chosen as the study model. After TPA and TPB enter into the live cells, the TK1 mRNA induces TPA and TPB to approach and activate the fluorescent aptamer, resulting in enhanced fluorescent signal in the presence of small molecules of DFHBI-1T. By this design, the high specificity label-free detection of nucleic acids was achieved with a detection limit of 1.34 nM. Confocal fluorescence imaging experiments had proved that this strategy could effectively distinguish the TK1 mRNA expression level between normal cell and Cancer cell. The developed method is expected to provide a new tool for early diagnosis of diseases and new drug development.

Keywords

Binary probes; DNA tetrahedron; Fluorogenic RNA aptamers; mRNA imaging.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-110251
    99.44%, RNA Aptamers Probe