2. Academic Validation
  3. Dissecting the early and late endosomal pathways of Singapore grouper iridovirus by single-particle tracking in living cells

Dissecting the early and late endosomal pathways of Singapore grouper iridovirus by single-particle tracking in living cells

  • Int J Biol Macromol. 2024 Jan;256(Pt 1):128336. doi: 10.1016/j.ijbiomac.2023.128336.
Liqun Wang 1,  Qiang Li 2,  Xiaozhi Wen 3,  Xinyue Zhang 3,  Shaowen Wang 4,  Qiwei Qin 5
Affiliations

Affiliations

  • 1 College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China; Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China.
  • 2 College of Oceanology and meteorology, Guangdong Ocean University, Zhanjiang 524088, China.
  • 3 College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
  • 4 College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China; Nansha-South China Agricultural University Fishery Research Institute, Guangzhou 511464, China. Electronic address: [email protected].
  • 5 College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China; Nansha-South China Agricultural University Fishery Research Institute, Guangzhou 511464, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai 519082, China. Electronic address: [email protected].
PMID: 38013078 DOI: 10.1016/j.ijbiomac.2023.128336
Abstract

Iridoviruses are large DNA viruses that infect a wide range of invertebrates and lower vertebrates, causing serious threats to ecological security and aquaculture industry worldwide. However, the mechanisms underlying intracellular transport of iridovirus remain unknown. In this study, the transport of Singapore grouper iridovirus (SGIV) in early endosomes (EEs) and late endosomes (LEs) was explored by single-particle tracking technology. SGIV employs EEs to move rapidly from the cell membrane to the nucleus, and this long-range transport is divided into "slow-fast-slow" stages. SGIV within LEs mainly underwent oscillatory movements near the nucleus. Furthermore, SGIV entered newly formed EEs and LEs, respectively, possibly based on the interaction between the viral major capsid protein and Rab5/Rab7. Importantly, interruption of EEs and LEs by the dominant negative mutants of Rab5 and Rab7 significantly inhibited the movement of SGIV, suggesting the important roles of Rab5 and Rab7 in virus transport. In addition, it seems that SGIV needs to enter clathrin-coated vesicles to move from actin to microtubules before EEs carry the virus moving along microtubules. Together, our results for the first time provide a model whereby iridovirus transport depending on EEs and LEs, helping to clarify the mechanism underlying iridovirus Infection, and provide a convenient tactic to investigate the dynamic Infection of large DNA virus.

Keywords

Early endosomes; Late endosomes; Singapore grouper iridovirus; Single-particle tracking technology.

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