1. Cytoskeleton Anti-infection Stem Cell/Wnt
  2. Arp2/3 Complex Antibiotic YAP
  3. Cytochalasin D

Cytochalasin D  (Synonyms: Zygosporin A; NSC 209835)

Cat. No.: HY-N6682 Purity: 99.76%
COA Handling Instructions

Cytochalasin D (Zygosporin A) is a potent actin polymerization inhibitor, could be derived from fungus. Cytochalasin D has cell-permeable activity. Cytochalasin D inhibits the G-actin–cofilin interaction by binding to G-actin. Cytochalasin D also inhibits the binding of cofilin to F-actin and decreases the rate of both actin polymerization and depolymerization in living cells. Cytochalasin D can reduce exosome release, in turn reducing the amount of survivin present in the tumour environment. Cytochalasin D induces phosphorylation and cytoplasmic retention of Yap.

For research use only. We do not sell to patients.

Cytochalasin D Chemical Structure

Cytochalasin D Chemical Structure

CAS No. : 22144-77-0

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 630 In-stock
Solution
10 mM * 1 mL in DMSO USD 630 In-stock
Solid
1 mg USD 250 In-stock
5 mg USD 560 In-stock
10 mg USD 920 In-stock
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Customer Review

Based on 39 publication(s) in Google Scholar

Top Publications Citing Use of Products

39 Publications Citing Use of MCE Cytochalasin D

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cytochalasin D (Zygosporin A) is a potent actin polymerization inhibitor, could be derived from fungus. Cytochalasin D has cell-permeable activity. Cytochalasin D inhibits the G-actin–cofilin interaction by binding to G-actin. Cytochalasin D also inhibits the binding of cofilin to F-actin and decreases the rate of both actin polymerization and depolymerization in living cells. Cytochalasin D can reduce exosome release, in turn reducing the amount of survivin present in the tumour environment. Cytochalasin D induces phosphorylation and cytoplasmic retention of Yap[1][2][3][4].

In Vitro

Cytochalasin D (3 and 10 μM; 30 min) causes retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures[1].
Cytochalasin D (0.3, 1, 3 and 10 μM; 30 min) concentration-dependently decreases the rate of actin depolymerization in COS-7 cells[1].
Cytochalasin D (1 μM; NIH3T3 cells) disrupts stress fibers, and induces phosphorylation of Yap, while cells maintain the original area and lost nuclear Yap localization[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line: COS-7 cells[1]
Concentration: 3 and 10 μM
Incubation Time: 30 min
Result: Caused retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures.

Western Blot Analysis

Cell Line: COS-7 cells expressing YFP-actin[1]
Concentration: 0.3, 1, 3 and 10 μM
Incubation Time: 30 min
Result: Concentration-dependently decreased the rate of actin depolymerization in COS-7 cells.
Clinical Trial
Molecular Weight

507.62

Formula

C30H37NO6

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C=C1[C@@H](O)[C@@]2([H])[C@@]3([C@H](OC(C)=O)/C=C/[C@](O)(C)C([C@@H](C)C/C=C/2)=O)[C@@]([C@H](CC4=CC=CC=C4)NC3=O)([H])[C@@H]1C

Structure Classification
Initial Source

molds

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (49.25 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9700 mL 9.8499 mL 19.6998 mL
5 mM 0.3940 mL 1.9700 mL 3.9400 mL
10 mM 0.1970 mL 0.9850 mL 1.9700 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: 99.76%

References
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Cytochalasin D Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Cytochalasin D
Cat. No.:
HY-N6682
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