1. Cytoskeleton Anti-infection Stem Cell/Wnt
  2. Arp2/3 Complex Antibiotic YAP
  3. Cytochalasin D

Cytochalasin D  (Synonyms: Zygosporin A; NSC 209835)

Cat. No.: HY-N6682 Purity: 99.76%
COA Handling Instructions

Cytochalasin D (Zygosporin A) is a potent actin polymerization inhibitor, could be derived from fungus. Cytochalasin D has cell-permeable activity. Cytochalasin D inhibits the G-actin–cofilin interaction by binding to G-actin. Cytochalasin D also inhibits the binding of cofilin to F-actin and decreases the rate of both actin polymerization and depolymerization in living cells. Cytochalasin D can reduce exosome release, in turn reducing the amount of survivin present in the tumour environment. Cytochalasin D induces phosphorylation and cytoplasmic retention of Yap.

For research use only. We do not sell to patients.

Cytochalasin D Chemical Structure

Cytochalasin D Chemical Structure

CAS No. : 22144-77-0

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 630 In-stock
Solution
10 mM * 1 mL in DMSO USD 630 In-stock
Solid
1 mg USD 250 In-stock
5 mg USD 560 In-stock
10 mg USD 920 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 40 publication(s) in Google Scholar

Top Publications Citing Use of Products

40 Publications Citing Use of MCE Cytochalasin D

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cytochalasin D (Zygosporin A) is a potent actin polymerization inhibitor, could be derived from fungus. Cytochalasin D has cell-permeable activity. Cytochalasin D inhibits the G-actin–cofilin interaction by binding to G-actin. Cytochalasin D also inhibits the binding of cofilin to F-actin and decreases the rate of both actin polymerization and depolymerization in living cells. Cytochalasin D can reduce exosome release, in turn reducing the amount of survivin present in the tumour environment. Cytochalasin D induces phosphorylation and cytoplasmic retention of Yap[1][2][3][4].

In Vitro

Cytochalasin D (3 and 10 μM; 30 min) causes retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures[1].
Cytochalasin D (0.3, 1, 3 and 10 μM; 30 min) concentration-dependently decreases the rate of actin depolymerization in COS-7 cells[1].
Cytochalasin D (1 μM; NIH3T3 cells) disrupts stress fibers, and induces phosphorylation of Yap, while cells maintain the original area and lost nuclear Yap localization[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line: COS-7 cells[1]
Concentration: 3 and 10 μM
Incubation Time: 30 min
Result: Caused retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures.

Western Blot Analysis

Cell Line: COS-7 cells expressing YFP-actin[1]
Concentration: 0.3, 1, 3 and 10 μM
Incubation Time: 30 min
Result: Concentration-dependently decreased the rate of actin depolymerization in COS-7 cells.
Clinical Trial
Molecular Weight

507.62

Formula

C30H37NO6

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C=C1[C@@H](O)[C@@]2([H])[C@@]3([C@H](OC(C)=O)/C=C/[C@](O)(C)C([C@@H](C)C/C=C/2)=O)[C@@]([C@H](CC4=CC=CC=C4)NC3=O)([H])[C@@H]1C

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (49.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9700 mL 9.8499 mL 19.6998 mL
5 mM 0.3940 mL 1.9700 mL 3.9400 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.76%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9700 mL 9.8499 mL 19.6998 mL 49.2494 mL
5 mM 0.3940 mL 1.9700 mL 3.9400 mL 9.8499 mL
10 mM 0.1970 mL 0.9850 mL 1.9700 mL 4.9249 mL
15 mM 0.1313 mL 0.6567 mL 1.3133 mL 3.2833 mL
20 mM 0.0985 mL 0.4925 mL 0.9850 mL 2.4625 mL
25 mM 0.0788 mL 0.3940 mL 0.7880 mL 1.9700 mL
30 mM 0.0657 mL 0.3283 mL 0.6567 mL 1.6416 mL
40 mM 0.0492 mL 0.2462 mL 0.4925 mL 1.2312 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Cytochalasin D Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Cytochalasin D
Cat. No.:
HY-N6682
Quantity:
MCE Japan Authorized Agent: