2. Cytoskeleton Anti-infection Stem Cell/Wnt
  3. Exosomes Arp2/3 Complex Antibiotic YAP
  4. Cytochalasin D

Cytochalasin D  (Synonyms: Zygosporin A; NSC 209835)

Cat. No.: HY-N6682 Purity: 99.0%
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Cytochalasin D (Zygosporin A) is a potent actin polymerization inhibitor, could be derived from fungus. Cytochalasin D has cell-permeable activity. Cytochalasin D inhibits the G-actin–cofilin interaction by binding to G-actin. Cytochalasin D also inhibits the binding of cofilin to F-actin and decreases the rate of both actin polymerization and depolymerization in living cells. Cytochalasin D can reduce exosome release, in turn reducing the amount of survivin present in the tumour environment. Cytochalasin D induces phosphorylation and cytoplasmic retention of Yap.

For research use only. We do not sell to patients.

CAS No. : 22144-77-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 91 publication(s) in Google Scholar

Cytochalasin D Related Antibodies

Top Publications Citing Use of Products

91 Publications Citing Use of MCE Cytochalasin D

Cell Imaging/Staining
RT-PCR

    Cytochalasin D purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Aug 3.

    Treatment with CytoD (1 μM, 6 h), MβCD (100 μM, 6 h), amiloride (50 μM, 6 h), or Pitstop 2 (50 μM, 6 h) significantly attenuated the intracellular green fluorescence of FITC/M-BSA@PSiNPs (A-B) and FITC/M-BSA (1C) in RAW 264.7 macrophages.

    Cytochalasin D purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Aug 3.

    CytoD （1μM ， 6h）， Mβ CD（100 μM， 6 h） inhibited the gene expression of IL-1β, IL-6, CCL2, IFN-β, CXCL10, and TNF-α in RAW 264.7 cells treated with (A) 100 μg /mL-M-BSA@PSiNPs, and (B) 100 μg /mL−M-BSA .

    Cytochalasin D purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Aug 16:21:110-128.

    Cytochalasin D (CytoD, 2 μM, 1 h) destroyed the cytoskeleton of BMSCs cultured in the daCO-DM modified scaffold.

    Cytochalasin D purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Aug 16:21:110-128.

    Cytochalasin D (CytoD, 2 μM, 1 h) reduced the AP activity of BMSCs cultured in the daCO-DM modified scaffold (Top: DMSO group; Bottom: CytoD group).

    Cytochalasin D purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Aug 16:21:110-128.

    Cytochalasin D (CytoD, 2 μM, 1 h) reduced the expression of osteoblast marker genes in BMSCs cultured in the daCO-DM modified scaffold.

    View All Antibiotic Isoform Specific Products:

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    View All YAP Isoform Specific Products:

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    YAP/TAZ YAP/TAZ-TEAD TEAD YAP1

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Cytochalasin D (Zygosporin A) is a potent actin polymerization inhibitor, could be derived from fungus. Cytochalasin D has cell-permeable activity. Cytochalasin D inhibits the G-actin–cofilin interaction by binding to G-actin. Cytochalasin D also inhibits the binding of cofilin to F-actin and decreases the rate of both actin polymerization and depolymerization in living cells. Cytochalasin D can reduce exosome release, in turn reducing the amount of survivin present in the tumour environment. Cytochalasin D induces phosphorylation and cytoplasmic retention of Yap[1][2][3][4].

    Cellular Effect
    Cell Line Type Value Description References
    MRC5 IC50
    2.36 μM
    Compound: 2; CytD
    Cytotoxicity against human MRC5 cells assessed as inhibition of cell growth incubated for 72 hrs by WST-1 assay
    Cytotoxicity against human MRC5 cells assessed as inhibition of cell growth incubated for 72 hrs by WST-1 assay
    		[PMID: 38283219]
    In Vitro

    Cytochalasin D (3 and 10 μM; 30 min) causes retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures[1].
    Cytochalasin D (0.3, 1, 3 and 10 μM; 30 min) concentration-dependently decreases the rate of actin depolymerization in COS-7 cells[1].
    Cytochalasin D (1 μM; NIH3T3 cells) disrupts stress fibers, and induces phosphorylation of Yap, while cells maintain the original area and lost nuclear Yap localization[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cytochalasin D Related Antibodies

    Western Blot Analysis

    Cell Line: COS-7 cells[1]
    Concentration: 3 and 10 μM
    Incubation Time: 30 min
    Result: Caused retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures.

    Western Blot Analysis

    Cell Line: COS-7 cells expressing YFP-actin[1]
    Concentration: 0.3, 1, 3 and 10 μM
    Incubation Time: 30 min
    Result: Concentration-dependently decreased the rate of actin depolymerization in COS-7 cells.
    Clinical Trial
    Molecular Weight

    507.62

    Formula

    C30H37NO6
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C=C1[C@@H](O)[C@@]2([H])[C@@]3([C@H](OC(C)=O)/C=C/[C@](O)(C)C([C@@H](C)C/C=C/2)=O)[C@@]([C@H](CC4=CC=CC=C4)NC3=O)([H])[C@@H]1C
    Structure Classification
    Initial Source

    molds
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (49.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9700 mL 9.8499 mL 19.6998 mL
    5 mM 0.3940 mL 1.9700 mL 3.9400 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.25 mg/mL (2.46 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.25 mg/mL (2.46 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.76%

    SDS (479 KB)

    COA (261 KB) HNMR (302 KB) RP-HPLC (241 KB) LCMS (86 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9700 mL 9.8499 mL 19.6998 mL 49.2494 mL
    5 mM 0.3940 mL 1.9700 mL 3.9400 mL 9.8499 mL
    10 mM 0.1970 mL 0.9850 mL 1.9700 mL 4.9249 mL
    15 mM 0.1313 mL 0.6567 mL 1.3133 mL 3.2833 mL
    20 mM 0.0985 mL 0.4925 mL 0.9850 mL 2.4625 mL
    25 mM 0.0788 mL 0.3940 mL 0.7880 mL 1.9700 mL
    30 mM 0.0657 mL 0.3283 mL 0.6567 mL 1.6416 mL
    40 mM 0.0492 mL 0.2462 mL 0.4925 mL 1.2312 mL
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    Cytochalasin D Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Cytochalasin D22144-77-0Zygosporin A NSC 209835NSC209835NSC 209835NSC-209835ExosomesArp2/3 ComplexAntibioticYAPActin-related protein 2/3 complex Yes-associated proteinCOS-7G-actinactin polymerizationactin depolymerizationphosphorylationInhibitorinhibitorinhibit

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