2. Cytoskeleton
  3. Arp2/3 Complex
  4. Latrunculin A

Latrunculin A (LAT-A), found in the red sea sponge Latrunculia magnifica, is a G-actin polymerization inhibitor. Latrunculin A binds to actin monomers and inhibits polymerization of actin with Kds of 0.1, 0.4, 4.7 μM and 0.19 μM for ATP-actin, ADP-Pi-actin, ADP-actin and G-actin, respectively. Latrunculin A has effective anti-metastatic properties for cancer research. Latrunculin A blocks cell migration.

For research use only. We do not sell to patients.

CAS No. : 76343-93-6

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100 μg (237.2 μM * 1 mL in Ethanol) In-stock

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Based on 12 publication(s) in Google Scholar

Latrunculin A Related Antibodies

Top Publications Citing Use of Products

    Latrunculin A purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Aug 28;e2203173.  [Abstract]

    The sEVs (50 μg) were cocultured with macrophages (1 × 106) for 24 h. The sEVsHNSCC derived from two HNSCC lines: SCC25 and HN6. The sEVshBMSC-NT5EOE derived from hBMSC cells with NT5E overexpression. Latrunculin A (Lat A, 30 μM; 24 h) was used as the inhibitor of sEVs uptaken showed downregulated phagocytosis. The percentage of pHrodo dyes of M2 macrophages was analyzed by flow cytometry.

    Latrunculin A purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Aug 28;e2203173.  [Abstract]

    The expression of cytokines also increased when macrophages were cocultured with sEVs derived from hBMSCs overexpressing CD73, whereas this trend was reversed by Latrunculin A (Lat A, 30 μM; 24 h).

    Latrunculin A purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Aug 28;e2203173.  [Abstract]

    Macrophages were cocultured with anti-CD73-FITC labelled sEVs from HNSCC cell lines control or RABAKO, CD73-GFP labelled sEVs from hBMSC treated with or without Latrunculin A (Lat A, 30 μM; 24 h) were cocultured with macrophages for 1 h, and Laser Scanning Confocal Microscopy was used to analyze the internalization of HNSCC-derived sEVs into macrophages (Scale bar = 25 μm).

    Latrunculin A purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Aug 28;e2203173.  [Abstract]

    Absence of CD73 in sEVs rescues immune suppression and restrains tumour growth in C3H/He mice aged 6–8 weeks. (a) Schematic of subcutaneous tumorigenesis in vivo experiment, followed with intratumoral injection of sEVs which were collected from SCC7, SCC7-Nt5eKO or SCC7-Nt5eOE cells grown in vitro. (b) The exhibition of isolated tumours. (c and d) The tumour weight and the time course of tumour growth in grams for 15 days postinjection with SCC7 or SCC7Rab27aKO cells with or without CD73 in sEVs. Latrunculin A (Lat A, 0.15 mg/kg; ip; every 2 days for 15 days) was used as inhibitor of sEVs uptaken.

    Latrunculin A purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Aug 28;e2203173.  [Abstract]

    Latrunculin A (Lat A, 0.15 mg/kg; ip; every 2 days for 15 days) was used as an inhibitor of sEVs uptake in C3H/He mice aged 6–8 weeks. Flow cytometry analysis for infiltration of Tregs and percentage of CD73+/PD-1+ Tregs in tumours.

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    Description

    Latrunculin A (LAT-A), found in the red sea sponge Latrunculia magnifica, is a G-actin polymerization inhibitor. Latrunculin A binds to actin monomers and inhibits polymerization of actin with Kds of 0.1, 0.4, 4.7 μM and 0.19 μM for ATP-actin, ADP-Pi-actin, ADP-actin and G-actin, respectively. Latrunculin A has effective anti-metastatic properties for cancer research. Latrunculin A blocks cell migration[1][2][3][4][5][6].

    IC50 & Target

    Kd: 0.1 μM (ATP-actin), 0.4 μM (ADP-Pi-actin), 4.7 μM (ADP-actin), 0.19 μM (G-actin)[2]
    Cellular Effect
    Cell Line Type Value Description References
    A549 IC50
    0.06 μg/mL
    Compound: 1
    Cytotoxicity against human A549 cells after 48 hrs by MTT assay
    Cytotoxicity against human A549 cells after 48 hrs by MTT assay
    		[PMID: 8350092]
    HCT-116 IC50
    1.1 μM
    Compound: 1, NSC-613011
    Cytotoxicity against human HCT116 cells after 3 days by trypan blue assay
    Cytotoxicity against human HCT116 cells after 3 days by trypan blue assay
    		[PMID: 18942825]
    HT-29 IC50
    0.06 μg/mL
    Compound: 1
    Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay
    Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay
    		[PMID: 8350092]
    HT-29 IC50
    0.08 μg/mL
    Compound: 10
    Antiproliferative activity against human HT-29 cells assessed as cell viability after 96 hrs by CellTiter-Glo luminescence assay
    Antiproliferative activity against human HT-29 cells assessed as cell viability after 96 hrs by CellTiter-Glo luminescence assay
    		[PMID: 22129061]
    MCF7 IC50
    0.48 μM
    Compound: 99a
    Anticancer activity against human MCF7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    Anticancer activity against human MCF7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    		[PMID: 33940466]
    MDA-MB-231 IC50
    4.19 μM
    Compound: 99a
    Anticancer activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    Anticancer activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    		[PMID: 33940466]
    MDA-MB-435 IC50
    0.04 μg/mL
    Compound: 10
    Antiproliferative activity against human MDA-MB-435 cells assessed as cell viability after 96 hrs by CellTiter-Glo luminescence assay
    Antiproliferative activity against human MDA-MB-435 cells assessed as cell viability after 96 hrs by CellTiter-Glo luminescence assay
    		[PMID: 22129061]
    MDA-MB-435 IC50
    2.8 μM
    Compound: 1, NSC-613011
    Cytotoxicity against human MDA-MB-435 cells 48 hrs by SRB assay
    Cytotoxicity against human MDA-MB-435 cells 48 hrs by SRB assay
    		[PMID: 18942825]
    P388 IC50
    4.1 μg/mL
    Compound: 1
    Cytotoxicity against mouse P388 cells after 48 hrs by MTT assay
    Cytotoxicity against mouse P388 cells after 48 hrs by MTT assay
    		[PMID: 8350092]
    P388 IC50
    4.1 μg/mL
    Compound: Latrunculin A
    Antiproliferative activity against mouse P388 cells
    Antiproliferative activity against mouse P388 cells
    		10.1021/np50082a019
    T47D IC50
    25 μM
    Compound: 1,Latrunculin A
    Inhibition of 1,10-phenanthroline-induced HIF1 activation in human T47D cells by cell based reporter gene assay
    Inhibition of 1,10-phenanthroline-induced HIF1 activation in human T47D cells by cell based reporter gene assay
    		[PMID: 18298079]
    T47D IC50
    6.7 μM
    Compound: 1,Latrunculin A
    Inhibition of hypoxia-induced HIF1 activation in human T47D cells by cell based reporter gene assay
    Inhibition of hypoxia-induced HIF1 activation in human T47D cells by cell based reporter gene assay
    		[PMID: 18298079]
    U-937 IC50
    0.07 μg/mL
    Compound: 10
    Antiproliferative activity against human U937 cells assessed as cell viability after 96 hrs by CellTiter-Glo luminescence assay
    Antiproliferative activity against human U937 cells assessed as cell viability after 96 hrs by CellTiter-Glo luminescence assay
    		[PMID: 22129061]
    In Vitro

    Latrunculin A (50-1000 nM) exhibits potent anti-invasive activity against human prostate cancer PC-3M cells, inhibits PC-3M-CT+ spheroids disaggregation and cell migration[3].
    ? Latrunculin A (3-30 μM) inhibits hypoxia-induced HIF-1 activation with an IC50 value of 6.7 μM in human breast carcinoma T47D cells[3].
    ? Latrunculin A (0-0.2 μM, 4 hours) has a significant inhibitory effect on HuR levels at high concentrations such as 0.2 μM in human hepatoma HepG2 cells while inhibits HuR only at 0.02 μM but no inhibitory effect at high concentrations in human hepatoma Huh7 cells[4].
    ? Latrunculin A (0.1 μM, 24 hours) can lead to a significant decrease in cell migration and has an inhibitory effect on cell proliferation in human hepatoma cell lines HepG2[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Latrunculin A Related Antibodies
    In Vivo

    Latrunculin A (intraperitoneal injection, 0.05 mg/kg, three doses in the first 20 days, 120 days) has strong antitumor effect in male BALB/c nude mice models infected with adenocarcinoma (MKN45) or carcinoma (NUGC-4)[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male BALB/c nude mice models infected with adenocarcinoma (MKN45) or carcinoma (NUGC-4)[5]
    Dosage: 0.05 mg/kg
    Administration: Intraperitoneal injection; three doses in the first 20 days; 120 days
    Result: Extended the mean life expectancy to 23.5 days comparing to control of 16 days in adenocarcinoma (MKN45) mice and the mean survival time was 42 days comparing to untreated of 31 days in carcinoma (NUGC-4) mice.
    Clinical Trial
    Molecular Weight

    421.55

    Formula

    C22H31NO5S
    CAS No.
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    O=C1SC[C@@H]([C@]2(O)O[C@]3([H])CC[C@H](C)/C=C\C=C\CC/C(C)=C\C(O[C@@](C3)([H])C2)=O)N1
    Structure Classification
    Initial Source

    red sea sponge Latrunculia magnifica
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Solution, -20°C, 2 years
    Purity & Documentation

    Purity: 98.9%

    SDS (394 KB)

    COA (255 KB)

    Handling Instructions (2659 KB)
    References
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    Latrunculin A Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Latrunculin A76343-93-6LAT-AArp2/3 ComplexActin-related protein 2/3 complexG-actin inhibitorATP-actinADP-Pi-actinADP-actinanti-canceranti-metastaticInhibitorinhibitorinhibit

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