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SMIFH2 

Cat. No.: HY-16931 Purity: 98.22%
Handling Instructions

SMIFH2 is a formin specific inhibitor. SMIFH2 inhibits actin polymerization by Formins and affects the actin cytoskeleton.

For research use only. We do not sell to patients.

SMIFH2 Chemical Structure

SMIFH2 Chemical Structure

CAS No. : 340316-62-3

Size Price Stock Quantity
5 mg USD 80 In-stock
Estimated Time of Arrival: December 31
10 mg USD 120 In-stock
Estimated Time of Arrival: December 31
50 mg USD 520 In-stock
Estimated Time of Arrival: December 31
100 mg USD 890 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

SMIFH2 is a formin specific inhibitor. SMIFH2 inhibits actin polymerization by Formins and affects the actin cytoskeleton[1].

IC50 & Target

Formin[1]

In Vitro

SMIFH2 (25 uM; 1-16 hours) induces dynamic cytoskeletal remodelling in U2OS cells[1].
SMIFH2 (25 μM) reduces p300, mDia2 and p53 levels in a proteasome-independent manner[1].
SMIFH2 reduces expression and activity of p53 through a post-transcriptional, proteasome-independent mechanism that influences remodelling of the cytoskeleton[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: 293T, A375, U2OS, and MDA-MB-231 cells
Concentration: 25 μM
Incubation Time: 5 hours for 293T, U2OS, MDA-MB-231 cells; 2.5 hours for A375 cells
Result: Downregulated mDia2, p53 and p300 protein levels.
Molecular Weight

377.21

Formula

C₁₅H₉BrN₂O₃S

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (331.38 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6510 mL 13.2552 mL 26.5104 mL
5 mM 0.5302 mL 2.6510 mL 5.3021 mL
10 mM 0.2651 mL 1.3255 mL 2.6510 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (5.51 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
References
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Product Name:
SMIFH2
Cat. No.:
HY-16931
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