2. Cytoskeleton
  3. Arp2/3 Complex
  4. SMIFH2

SMIFH2 is a formin specific inhibitor. SMIFH2 inhibits actin polymerization by Formins and affects the actin cytoskeleton.

For research use only. We do not sell to patients.

CAS No. : 340316-62-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 9 publication(s) in Google Scholar

SMIFH2 Related Antibodies

Top Publications Citing Use of Products

    SMIFH2 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jan 20:140112.  [Abstract]

    SMIFH2 (1-50 μM; 4 h) treatment resulted in a decrease in PRV-GFP proliferation, as indicated by a lower percentage of GFP-positive cells in PK-15 cells.

    SMIFH2 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jan 20:140112.  [Abstract]

    SMIFH2 (5-50 μM; 4 h) caused dose-dependent inhibition of PRV replication in PK-15 cells.

    SMIFH2 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jan 20:140112.  [Abstract]

    SMIFH2 (1-50 μM; 4 h) inhibited DIAPH1 and diminished the PRV viral progeny production.

    SMIFH2 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jan 20:140112.  [Abstract]

    SMIFH2 (50 μM; 4 h) decreased the level of acetylated-α-tubulin in PK-15 cells.

    SMIFH2 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jan 20:140112.  [Abstract]

    SMIFH2 (50 μM; 4 h) decreased actin levels and the formation of thick, long actin stress fibers, resulting in more thin and short microfilaments in PK-15 cells.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    SMIFH2 is a formin specific inhibitor. SMIFH2 inhibits actin polymerization by Formins and affects the actin cytoskeleton[1].

    IC50 & Target

    Formin[1]
    In Vitro

    SMIFH2 (25 uM; 1-16 hours) induces dynamic cytoskeletal remodelling in U2OS cells[1].
    SMIFH2 (25 μM) reduces p300, mDia2 and p53 levels in a proteasome-independent manner[1].
    SMIFH2 reduces expression and activity of p53 through a post-transcriptional, proteasome-independent mechanism that influences remodelling of the cytoskeleton[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    SMIFH2 Related Antibodies

    Western Blot Analysis[1]

    Cell Line: 293T, A375, U2OS, and MDA-MB-231 cells
    Concentration: 25 μM
    Incubation Time: 5 hours for 293T, U2OS, MDA-MB-231 cells; 2.5 hours for A375 cells
    Result: Downregulated mDia2, p53 and p300 protein levels.
    Molecular Weight

    377.21

    Formula

    C15H9BrN2O3S
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to green yellow

    SMILES

    O=C(/C(C(N1C2=CC=CC(Br)=C2)=O)=C\C3=CC=CO3)NC1=S
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (331.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6510 mL 13.2552 mL 26.5104 mL
    5 mM 0.5302 mL 2.6510 mL 5.3021 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.08 mg/mL (5.51 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.08%

    SDS (393 KB)

    COA (250 KB) HNMR (263 KB) RP-HPLC (248 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.6510 mL 13.2552 mL 26.5104 mL 66.2761 mL
    5 mM 0.5302 mL 2.6510 mL 5.3021 mL 13.2552 mL
    10 mM 0.2651 mL 1.3255 mL 2.6510 mL 6.6276 mL
    15 mM 0.1767 mL 0.8837 mL 1.7674 mL 4.4184 mL
    20 mM 0.1326 mL 0.6628 mL 1.3255 mL 3.3138 mL
    25 mM 0.1060 mL 0.5302 mL 1.0604 mL 2.6510 mL
    30 mM 0.0884 mL 0.4418 mL 0.8837 mL 2.2092 mL
    40 mM 0.0663 mL 0.3314 mL 0.6628 mL 1.6569 mL
    50 mM 0.0530 mL 0.2651 mL 0.5302 mL 1.3255 mL
    60 mM 0.0442 mL 0.2209 mL 0.4418 mL 1.1046 mL
    80 mM 0.0331 mL 0.1657 mL 0.3314 mL 0.8285 mL
    100 mM 0.0265 mL 0.1326 mL 0.2651 mL 0.6628 mL
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    SMIFH2 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    SMIFH2340316-62-3SMIFH 2SMIFH-2Arp2/3 ComplexActin-related protein 2/3 complexHeLalungadenocarcinomaepithelialbreastcarcinomaforminactinpolymerizationcytoskeletonInhibitorinhibitorinhibit

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    Cat. No.:
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