2. Cytoskeleton Anti-infection
  3. Arp2/3 Complex HIV
  4. CK-666

CK-666 is a cell-permeable actin-related protein Arp2/3 complex inhibitor (IC50=12 μM). CK-666 binds to Arp2/3 complex, stabilizes the inactive state of the complex, blocking movement of the Arp2 and Arp3 subunits into the activated filament-like (short pitch) conformation.

For research use only. We do not sell to patients.

CAS No. : 442633-00-3

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Customer Review

Based on 25 publication(s) in Google Scholar

CK-666 Related Antibodies

Top Publications Citing Use of Products

    CK-666 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Aug;14(8):e70146.  [Abstract]

    CK666 (25 μM, 24.5 h) reduced the uptake of S-EMs by VSMCs.

    CK-666 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Aug;14(8):e70146.  [Abstract]

    CK666 (25 μM, 24.5 h) inhibited the S-EMs-induced proliferation of VSMCs from normotensive Wistar-Kyoto rats and spontaneously hypertensive rats.

    CK-666 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Aug;14(8):e70146.  [Abstract]

    CK666 (25 μM, 24.5 h) inhibited the S-EMs-induced migration of VSMCs from normotensive Wistar-Kyoto rats and spontaneously hypertensive rats.

    CK-666 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 17:e00358.  [Abstract]

    CK666 (2.5 μg/mL, 4 days) reduced F-actin polymerization in E15.5 fetal mice.

    CK-666 purchased from MedChemExpress. Usage Cited in: NPJ Parkinsons Dis. 2024 Apr 9;10(1):80.  [Abstract]

    CK666 (100 μM, 24 h) reduced the F-actin/G-actin ratio in doxycycline-treated iPSC-derived neurons.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    CK-666 is a cell-permeable actin-related protein Arp2/3 complex inhibitor (IC50=12 μM). CK-666 binds to Arp2/3 complex, stabilizes the inactive state of the complex, blocking movement of the Arp2 and Arp3 subunits into the activated filament-like (short pitch) conformation[1][2].

    IC50 & Target[2]

    HIV-1

     

    In Vitro

    CK-666 (100 μM) significantly reduces the number of filopodia on the cell surface of treated trabecular meshwork (TM) cells as well as reduces the length of those filopodia that are present[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    CK-666 Related Antibodies
    Molecular Weight

    296.34

    Formula

    C18H17FN2O
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(NCCC1=C(C)NC2=C1C=CC=C2)C3=CC=CC=C3F
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (421.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3745 mL 16.8725 mL 33.7450 mL
    5 mM 0.6749 mL 3.3745 mL 6.7490 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 12.5 mg/mL (42.18 mM); Clear solution

      This protocol yields a clear solution of ≥ 12.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (125.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 12.5 mg/mL (42.18 mM); Clear solution

      This protocol yields a clear solution of ≥ 12.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (125.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.3745 mL 16.8725 mL 33.7450 mL 84.3626 mL
    5 mM 0.6749 mL 3.3745 mL 6.7490 mL 16.8725 mL
    10 mM 0.3375 mL 1.6873 mL 3.3745 mL 8.4363 mL
    15 mM 0.2250 mL 1.1248 mL 2.2497 mL 5.6242 mL
    20 mM 0.1687 mL 0.8436 mL 1.6873 mL 4.2181 mL
    25 mM 0.1350 mL 0.6749 mL 1.3498 mL 3.3745 mL
    30 mM 0.1125 mL 0.5624 mL 1.1248 mL 2.8121 mL
    40 mM 0.0844 mL 0.4218 mL 0.8436 mL 2.1091 mL
    50 mM 0.0675 mL 0.3375 mL 0.6749 mL 1.6873 mL
    60 mM 0.0562 mL 0.2812 mL 0.5624 mL 1.4060 mL
    80 mM 0.0422 mL 0.2109 mL 0.4218 mL 1.0545 mL
    100 mM 0.0337 mL 0.1687 mL 0.3375 mL 0.8436 mL
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    CK-666 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    CK-666442633-00-3CK666CK 666Arp2/3 ComplexHIVActin-related protein 2/3 complex Human immunodeficiency virustrabecularmeshworkallostericeffectoractivestateInhibitorinhibitorinhibit

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