1. Cytoskeleton
  2. Arp2/3 Complex
  3. CK-666

CK-666 

Cat. No.: HY-16926 Purity: 99.63%
Handling Instructions

CK-666 is a cell-permeable actin-related protein Arp2/3 complex inhibitor (IC50=12 μM). CK-666 binds to Arp2/3 complex, stabilizes the inactive state of the complex, blocking movement of the Arp2 and Arp3 subunits into the activated filament-like (short pitch) conformation.

For research use only. We do not sell to patients.

CK-666 Chemical Structure

CK-666 Chemical Structure

CAS No. : 442633-00-3

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 99 In-stock
Estimated Time of Arrival: December 31
5 mg USD 90 In-stock
Estimated Time of Arrival: December 31
10 mg USD 160 In-stock
Estimated Time of Arrival: December 31
50 mg USD 650 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 2 publication(s) in Google Scholar

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Description

CK-666 is a cell-permeable actin-related protein Arp2/3 complex inhibitor (IC50=12 μM). CK-666 binds to Arp2/3 complex, stabilizes the inactive state of the complex, blocking movement of the Arp2 and Arp3 subunits into the activated filament-like (short pitch) conformation[1][2].

IC50 & Target

Arp2/3 complex[1]

In Vitro

CK-666 (100 μM) significantly reduces the number of filopodia on the cell surface of treated trabecular meshwork (TM) cells as well as reduces the length of those filopodia that are present[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

296.34

Formula

C₁₈H₁₇FN₂O

CAS No.

442633-00-3

SMILES

O=C(NCCC1=C(C)NC2=C1C=CC=C2)C3=CC=CC=C3F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (421.81 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3745 mL 16.8725 mL 33.7450 mL
5 mM 0.6749 mL 3.3745 mL 6.7490 mL
10 mM 0.3375 mL 1.6873 mL 3.3745 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 12.5 mg/mL (42.18 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 12.5 mg/mL (42.18 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 12.5 mg/mL (42.18 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

CK-666CK666CK 666Arp2/3 ComplexActin-related protein 2/3 complextrabecularmeshworkallostericeffectoractivestateInhibitorinhibitorinhibit

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Product Name:
CK-666
Cat. No.:
HY-16926
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