2. Academic Validation
  3. In situ imaging of mRNA transcripts based on split-aptamer and split protein in living cells

In situ imaging of mRNA transcripts based on split-aptamer and split protein in living cells

  • RSC Adv. 2024 Mar 26;14(15):10146-10151. doi: 10.1039/d3ra08475b.
Yan Peng 1 2,  Mengqi Li 1,  Fuyu Gong 1 2,  Xiaofei Liu 1 3,  Jirou Xiong 1,  Keran Wang 1
Affiliations

Affiliations

  • 1 The Affiliated First Hospital of Fuyang Normal University, Fuyang Normal University Fuyang Anhui 236037 P.R. China [email protected] [email protected].
  • 2 Fuyang Women and Children's Hospital Fuyang Anhui 236037 P.R. China.
  • 3 Fuyang People's Hospital Fuyang Anhui 236000 P.R. China.
PMID: 38544938 DOI: 10.1039/d3ra08475b
Abstract

Messenger RNA (mRNA) is an essential component of cell development and growth. However, the detection of endogenous mRNA in living cells is currently limited. To address this issue, we have developed a novel strategy that comprises split-aptamer and split fluorescent protein dual-color, "turn-on" probes that specifically target mRNA with complementary sequences. Our split-aptamer and split-protein-initiated fluorescence complementation (sAPiFC) approach for live-cell imaging has demonstrated selectivity, stability, and capability for targeting various mRNAs.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-110251
    98.12%, RNA Aptamers Probe