2. Academic Validation
  3. CircInpp5b Ameliorates Renal Interstitial Fibrosis by Promoting the Lysosomal Degradation of DDX1

CircInpp5b Ameliorates Renal Interstitial Fibrosis by Promoting the Lysosomal Degradation of DDX1

  • Biomolecules. 2024 May 23;14(6):613. doi: 10.3390/biom14060613.
Xi Fang 1 2,  Chengyuan Tang 1 2,  Dong Zeng 1 2,  Yi Shan 1 2,  Qianfang Liu 1 2,  Xuemin Yin 1 2,  Ying Li 1 2
Affiliations

Affiliations

  • 1 Department of Nephrology, The Second Xiangya Hospital, Central South University, Changsha 410011, China.
  • 2 Key Laboratory of Kidney Disease and Blood Purification in Hunan Province, Changsha 410011, China.
PMID: 38927017 DOI: 10.3390/biom14060613
Abstract

Renal interstitial fibrosis (RIF) is a classic pathophysiological process of chronic kidney disease (CKD). However, the mechanisms underlying RIF remain unclear. The present study found that a novel circular RNA, cirInpp5b, might be involved in RIF by high-throughput Sequencing. Subsequent experiments revealed that circInpp5b was reduced in UUO mouse kidney tissues and TGF-β1-treated proximal tubular cells. The overexpression of circInpp5b inhibited RIF in UUO mice and prevented extracellular matrix (ECM) deposition in TGF-β1-treated proximal tubular cells. Furthermore, overexpression of circInpp5b down-regulated the protein level of DDX1. Mechanistically, circInpp5b bound to the DDX1 protein and promoted its lysosomal degradation. Collectively, the findings of our study demonstrate that circInpp5b ameliorates RIF by binding to the DDX1 protein and promoting its lysosomal degradation.

Keywords

DDX1; circInpp5b; circRNA; lysosomal degradation; renal interstitial fibrosis.

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