2. Academic Validation
  3. Developing Device of Death Operation (DODO) to Detect Apoptosis in 2D and 3D Cultures

Developing Device of Death Operation (DODO) to Detect Apoptosis in 2D and 3D Cultures

  • Cells. 2024 Jul 20;13(14):1224. doi: 10.3390/cells13141224.
Ziheng Zhang 1,  Zhe Sun 1,  Ji-Long Liu 1 2
Affiliations

Affiliations

  • 1 School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • 2 Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3PT, UK.
PMID: 39056805 DOI: 10.3390/cells13141224
Abstract

The real-time detection of intracellular biological processes by encoded sensors has broad application prospects. Here, we developed a degron-based modular reporting system, the Device of Death Operation (DODO), that can monitor various biological processes. The DODO system consists of a "reporter", an "inductor", and a "degron". After zymogen activation and cleavage, the degron will be released from the "reporter", which eventually leads to the stabilization of the "reporter", and can be detected. By replacing different "inductors" and "reporters", a series of biological processes can be reported through various signals. The system can effectively report the existence of TEV protease. To prove this concept, we successfully applied the DODO system to report Apoptosis in 2D and 3D cultures. In addition, the reporter based on degron will help to design protease reporters Other than Caspase.

Keywords

2D culture; 3D culture; DODO; apoptosis; degron.

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