2. Academic Validation
  3. Generation of an induced pluripotent stem cell line (CSBZZUi003-A) from a healthy male volunteer

Generation of an induced pluripotent stem cell line (CSBZZUi003-A) from a healthy male volunteer

  • Stem Cell Res. 2025 Dec:89:103867. doi: 10.1016/j.scr.2025.103867.
Wen-Xiu Li 1,  Dian-Dian Wang 1,  Hui-Fang Sun 2,  Ying-Jie Zhang 1,  Dan-Hua Liu 1,  Jia-Kang Li 1,  Pei-Pei Liu 3,  Shu-Ang Li 3,  Xiao-Yan Meng 4,  Jian-Sheng Kang 5
Affiliations

Affiliations

  • 1 Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450001, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China.
  • 2 Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China.
  • 3 Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
  • 4 Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450001, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China. Electronic address: [email protected].
  • 5 Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: [email protected].
PMID: 41218450 DOI: 10.1016/j.scr.2025.103867
Abstract

Primary fibroblasts were obtained from the dermal tissue from a healthy 50-year-old male donor and reprogrammed into induced pluripotent stem cells (iPSCs) using electroporation of reprogramming plasmids. The undifferentiated state of iPSCs was confirmed by immunofluorescence and flow cytometry analysis of stem cell markers, including TRA-1-60, Nanog, SOX2, and OCT4. The cells exhibited a normal karyotype. To evaluate their differentiation potential, the iPSCs were subcutaneously injected into immunodeficient mice, resulting in teratoma formation containing representative tissues of all three germ layers: endoderm, mesoderm, and ectoderm.

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