2. Academic Validation
  3. Generation of integration-free human induced pluripotent stem cell line MUi040-A derived from CD34 + hematopoietic stem cells of a patient with constitutional pericentric inversion of chromosome 9

Generation of integration-free human induced pluripotent stem cell line MUi040-A derived from CD34 + hematopoietic stem cells of a patient with constitutional pericentric inversion of chromosome 9

  • Stem Cell Res. 2025 Dec:89:103866. doi: 10.1016/j.scr.2025.103866.
Aung Khine Linn 1,  Phetcharat Phanthong 2,  Yongyut Pewkliang 3,  Wanida Laosee 2,  Pirut Tong-Ngam 4,  Alisa Tubsuwan 4,  Objoon Trachoo 5,  Nithi Asavapanumas 6,  Suparerk Borwornpinyo 7,  Suradej Hongeng 3,  Teerapat Yingchoncharoen 8
Affiliations

Affiliations

  • 1 Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, Bangkok 10400 Thailand.
  • 2 Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
  • 3 Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
  • 4 Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
  • 5 Department of Medical Genetics, College of Health Sciences, VinUniversity, Hanoi 10000, Vietnam; Center for Global Health, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA; Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
  • 6 Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bang Pla, Bang Phli, Samut Prakan 10540, Thailand.
  • 7 Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, Bangkok 10400 Thailand; Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
  • 8 Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand. Electronic address: [email protected].
PMID: 41223746 DOI: 10.1016/j.scr.2025.103866
Abstract

The MUi040-A human induced pluripotent stem cell (hiPSC) line was established from CD34 + hematopoietic stem cells of a male patient with senile amyloidosis. Patient carries a unique chromosome inversion at bands 9p12 and 9q13, which is one of the most common variants of the normal karyotype of chromosome 9 (inv[9]). This hiPSC line maintains an identical karyotype to that of the parental cells. MUi040-A expressed pluripotency markers and demonstrated the capacity to differentiate into all three germ layers. This cell line offers a valuable in vitro for studying the pathophysiology of senile systemic amyloidosis and for evaluating potential therapeutic approaches.

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