2. Academic Validation
  3. Light-Up Nanostructures with Allosterically Controlled Fluorogenic DNA Aptamers

Light-Up Nanostructures with Allosterically Controlled Fluorogenic DNA Aptamers

  • Adv Mater. 2025 Nov 17:e14306. doi: 10.1002/adma.202514306.
Tianqing Zhang 1,  Xinmin Qian 2 3 4,  Jiayi Zhang 5 6,  Huangchen Cui 5 6,  Tanxi Bai 5 6,  Wen Wang 7 8 9,  Yuxin Wang 5 6,  Zhaoli Gao 10,  Da Han 1,  Wenwen Zeng 2 3 4,  Bryan Wei 5 6
Affiliations

Affiliations

  • 1 Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou, 310022, China.
  • 2 Tsinghua-Peking Joint Center for Life Sciences, Beijing, 100084, China.
  • 3 School of Basic Medical Sciences and Institute for Immunology, Tsinghua University, Beijing, 100084, China.
  • 4 Beijing Key Laboratory of Immunological Research of Allergy, Beijing, 100084, China.
  • 5 School of Life Sciences, Tsinghua University, Beijing, 100084, China.
  • 6 Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China.
  • 7 Beijng Key Laboratory of Biomolecular Data Storage, BGI Research, Beijing, 102601, China.
  • 8 State Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen, 518083, China.
  • 9 BGI Research, Changzhou, 213299, China.
  • 10 Department of Biomedical Engineering, Chinese University of Hong Kong, Hong Kong, 999077, China.
PMID: 41243624 DOI: 10.1002/adma.202514306
Abstract

In this study, a systematic approach is presented for the rational design of synthetic allosteric DNA Aptamers. This methodology enables precise control over the allosteric ON-OFF transition in fluorescent DNA Aptamers, allowing for the engineering of Aptamers with highly tunable fluorescent properties. When combined with toehold-mediated strand displacement, a series of allosteric Aptamers is developed in which the target sequence functions as a specific allosteric modulator. Furthermore, these Aptamers are applied in synthetic DNA computing and in the construction of responsive nanostructures that light up upon activation.

Keywords

DNA computing; DNA nanotechnology; allosteric regulation; fluorogenic DNA aptamer.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-110251
    99.44%, RNA Aptamers Probe