2. Academic Validation
  3. Inhalable targeted CD8 + T cell nanoparticles loaded ferroptosis inhibitor ferrostatin-1 alleviate acute lung injury

Inhalable targeted CD8 + T cell nanoparticles loaded ferroptosis inhibitor ferrostatin-1 alleviate acute lung injury

  • J Nanobiotechnology. 2025 Nov 20;23(1):723. doi: 10.1186/s12951-025-03723-1.
Chunhua Ma # 1 2,  Zhaocong Yang # 3,  Jing Wang # 4,  Xuemei Li 5 6,  Wei Zhou 7 8,  Tao Li 9,  Liangming Liu 10
Affiliations

Affiliations

  • 1 Women and Children's Hospital of Chongqing Medical University, 400016, Chongqing, China.
  • 2 Chongqing Health Center for Women and Children, Chongqing, China, 400016, Chongqing, P. R. China.
  • 3 Children's Hospital of Nanjing Medical University, Nanjing, 210009, China.
  • 4 School of Biology and Food Engineering, Institute of Pharmaceutical Pharmacology Research Center, Suzhou University, 234099, Anhui, Suzhou, China.
  • 5 Women and Children's Hospital of Chongqing Medical University, 400016, Chongqing, China. [email protected].
  • 6 Chongqing Health Center for Women and Children, Chongqing, China, 400016, Chongqing, P. R. China. [email protected].
  • 7 Women and Children's Hospital of Chongqing Medical University, 400016, Chongqing, China. [email protected].
  • 8 Chongqing Health Center for Women and Children, Chongqing, China, 400016, Chongqing, P. R. China. [email protected].
  • 9 State Key Laboratory of Trauma, Burns and Combined Injury, Shock and Transfusion Research Department of Army Medical Center, Army Medical University, 400042, Chongqing, P. R. China. [email protected].
  • 10 State Key Laboratory of Trauma, Burns and Combined Injury, Shock and Transfusion Research Department of Army Medical Center, Army Medical University, 400042, Chongqing, P. R. China. [email protected].
  • # Contributed equally.
PMID: 41261431 DOI: 10.1186/s12951-025-03723-1
Abstract

Acute lung injury (ALI) is a common clinical complication of sepsis and Ferroptosis plays an important role in ALI. The aim of this study was to evaluate the effects of CD8 antibodies modified PD-1 engineered CD8+ T lymphocytes (CTL) membrane loaded poly lactic-co-glycolic acid (PLGA)/Ferroptosis inhibitor ferrostatin-1 (Fer-1) (CD8@PD-1-CTL-M@PLGA/Fer-1) on acute lung injury (ALI). High expression PD-1 CD8 + T (CTL) was established and the membrane (PD-1-CTL-M) was extracted, CD8 monoclonal antibody modified PD-1-CTL-M was used to enhance the targeting capability of PLGA/Fer-1 nanoparticles to target CD8 + T (CTL). ALI model was established by lipopolysaccharide (LPS) with mice and CD8 + T (CTL). The results showed that CD8@PD-1-CTL-M significantly enhanced the protective effects of PLGA/Fer-1 on ALI and targetability of PLGA/Fer-1 nanoparticles on CD8 + T (CTL). The mechanism was related to CD8@PD-1-CTL-M@PLGA/Fer-1 targeting regulation of PI3K/Akt and MAPK signal pathway in CD8 + T (CTL). This study provided a new treatment method for ALI.

Keywords

Acute lung injury; CD8; CD8 + T (CTL); Ferroptosis; PD-1.

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