2. Academic Validation
  3. Combining sodium chloride nanoparticle osmolarity spikes with irinotecan chemotherapy to amplify oxidative stress in the treatment of colorectal cancer

Combining sodium chloride nanoparticle osmolarity spikes with irinotecan chemotherapy to amplify oxidative stress in the treatment of colorectal cancer

  • Colloids Surf B Biointerfaces. 2025 Nov 19:259:115294. doi: 10.1016/j.colsurfb.2025.115294.
Amit Shrestha 1,  Prajeena Karmacharya 2,  Smala Shrestha 2,  Sunil Mishra 2,  Roopa Hirachand Patil 2,  Beomsu Kim 2,  Huy Xuan Luong 3,  Sae Kwang Ku 4,  Jeonghwan Kim 2,  Basavaraj Rudragouda Patil 5,  Jong Oh Kim 6
Affiliations

Affiliations

  • 1 College of Pharmacy, Yeungnam University, Daehakro-280, Gyeongsan 38541, Republic of Korea; Department of Pharmacy, CiST College, Pokhara University, Kathmandu 44600, Nepal.
  • 2 College of Pharmacy, Yeungnam University, Daehakro-280, Gyeongsan 38541, Republic of Korea.
  • 3 Faculty of Pharmacy, Phenikaa University, Hanoi 12116, Vietnam.
  • 4 College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
  • 5 Department of BioNano Technology, Gachon University, 1342 Seongnam-Daero, Sujeong-Gu, Gyeonggi-Do 13120, Republic of Korea. Electronic address: [email protected].
  • 6 College of Pharmacy, Yeungnam University, Daehakro-280, Gyeongsan 38541, Republic of Korea. Electronic address: [email protected].
PMID: 41297221 DOI: 10.1016/j.colsurfb.2025.115294
Abstract

Sodium chloride nanoparticles (SCNPs) have potential utility in Anticancer treatment. When these nanoparticles enter a cell through endocytosis and disrupt cellular homeostasis, an osmotic spike is triggered inside the cell. This causes the generation of Reactive Oxygen Species (ROS), ultimately leading to tumor cell death. We developed SCNPs and coated them with PLGA to create SC@PLGA-NPs. These were then encapsulated in lipids to produce SC@PLGA-LPs. We then incorporated irinotecan (CPT-11), resulting in the creation of SC@PLGA/CPT-11-LPs for use in the treatment of colorectal Cancer. The in vitro data confirmed the successful encapsulation and release of SCNPs. This resulted in increased osmotic pressure and ROS generation. The addition of CPT-11 to SCNPs further increased ROS production. Finally, these effects, combined with the Topoisomerase I inhibitory mechanism of CPT-11, resulted in the Apoptosis of HT-29 cells. Intratumoral injection of SC@PLGA/CPT-11-LPs into an HT-29 xenograft mouse model provided in vivo confirmation that this is a reliable and effective method for the delivery of NPs and drugs.

Keywords

Cancer; Irinotecan (CPT-11); Osmosis; ROS; Sodium chloride nanoparticles.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-15534
    99.0%, Mitochondrial Membrane Potential Probe