2. Academic Validation
  3. The establishment of a GPD1L knockout human embryonic stem cell line (WAe009-A-80) using the CRISPR/Cas9 system

The establishment of a GPD1L knockout human embryonic stem cell line (WAe009-A-80) using the CRISPR/Cas9 system

  • Stem Cell Res. 2026 Mar:91:103910. doi: 10.1016/j.scr.2026.103910.
Tao Dong 1,  Yan Zhao 2,  Hai-Feng Jin 3,  Hong-Ming Pan 4,  Li-Ling Yue 5,  Yan Lin 4,  Lei Shen 6
Affiliations

Affiliations

  • 1 Department of Anatomy, Histology and Embryology, Basic Medicine School, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China; Heilongjiang Provincial Key Laboratory of Food & Medicine Homology and Metabolic Disease Prevention, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China. Electronic address: [email protected].
  • 2 College of Life Science and Agroforestry, Qiqihar University, Qiqihar, Heilongjiang 161006, China.
  • 3 Department of Anatomy, Histology and Embryology, Basic Medicine School, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China; Heilongjiang Provincial Key Laboratory of Food & Medicine Homology and Metabolic Disease Prevention, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China.
  • 4 Heilongjiang Provincial Key Laboratory of Food & Medicine Homology and Metabolic Disease Prevention, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China.
  • 5 Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Heilongjiang 161006, China.
  • 6 Heilongjiang Provincial Key Laboratory of Food & Medicine Homology and Metabolic Disease Prevention, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China. Electronic address: [email protected].
PMID: 41539085 DOI: 10.1016/j.scr.2026.103910
Abstract

The GPD1L gene is located on 3p22.3. It encodes the glycerol phosphate dehydrogenase 1-like protein with homology to glycerol phosphate dehydrogenase (GPD1L), but the function of this enzyme is unclear. Mutations in GPD1L have been associated with BrS (Brugada syndrome) and SIDS (sudden infant death syndrome) and reduce Na+ inward current through an unknown mechanism in human cardiomyocytes. Here, a GPD1L knockout human embryonic stem cell line was generated using CRISPR/Cas9 system. The GPD1L knockout human embryonic stem cell maintains the pluripotency, differentiation into three germ layers, forming normal EBs.

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