2. Academic Validation
  3. Dandelion (Taraxacum mongolicum Hand. -Mazz.) extract inhibits triple-negative breast cancer by inducing ferroptosis via NCOA4-mediated ferritinophagy

Dandelion (Taraxacum mongolicum Hand. -Mazz.) extract inhibits triple-negative breast cancer by inducing ferroptosis via NCOA4-mediated ferritinophagy

  • J Ethnopharmacol. 2026 May 23:363:121393. doi: 10.1016/j.jep.2026.121393.
Zhengwang Guo 1,  Huifeng Hao 2,  Xinxin Deng 1,  Yanna Jiao 2,  Bin Dong 3,  Dong Xue 4,  Shuyan Han 5
Affiliations

Affiliations

  • 1 Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, 100191, China; Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Integration of Chinese and Western Medicine, Peking University Cancer Hospital & Institute, Beijing, 100142, China.
  • 2 Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Integration of Chinese and Western Medicine, Peking University Cancer Hospital & Institute, Beijing, 100142, China.
  • 3 Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Central Laboratory, Peking University Cancer Hospital & Institute, Beijing, 100142, China.
  • 4 Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Integration of Chinese and Western Medicine, Peking University Cancer Hospital & Institute, Beijing, 100142, China. Electronic address: [email protected].
  • 5 Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, 100191, China; Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Integration of Chinese and Western Medicine, Peking University Cancer Hospital & Institute, Beijing, 100142, China. Electronic address: [email protected].
PMID: 41707813 DOI: 10.1016/j.jep.2026.121393
Abstract

Ethnopharmacological relevance: Triple-negative breast Cancer (TNBC) has a poor clinical prognosis and shows resistance to traditional treatments. Ferroptosis is a potential therapeutic strategy for TNBC. Although dandelion extract has demonstrated anti-tumor effects against TNBC, its role in regulating Ferroptosis has not been elucidated.

Aim of the study: This study aimed to investigate the role and mechanisms of dandelion extract in inhibiting TNBC via the induction of Ferroptosis.

Materials and methods: Cell viability and cell death were detected by CCK-8 assay and PI/Hoechst 33342 dual staining, respectively. Proteomic profiling was used to identify the mechanisms of dandelion extract-induced cell death. Ferroptosis was assessed by measuring key hallmarks, including Fe2+ accumulation, lipid peroxidation, mitochondrial dysfunction, and ultrastructural changes. Mechanistic validation was achieved through target-specific interference and subsequent biomolecular analysis.

Results: Dandelion extract selectively induced cell death in TNBC cells without affecting normal MCF-10A cells. Proteomic and experimental evidence indicates that dandelion extract induces Ferroptosis in TNBC cells, characterized by elevated Fe2+, ROS, lipid peroxidation, MDA levels, and mitochondrial dysfunction. Crucially, Ferroptosis inhibitors attenuated the inhibitory effect of dandelion extract on TNBC cell viability, confirming Ferroptosis as the primary cell death mechanism. Mechanistic studies have demonstrated that dandelion extract promoted Ferroptosis in TNBC cells both in vitro and in vivo through NCOA4-mediated ferritinophagy.

Conclusions: This study demonstrates that dandelion extract induces Ferroptosis in TNBC cells both in vitro and in vivo by regulating ferritinophagy mediated by the NCOA4-FTH1 axis, thus suggesting novel therapeutic strategies for TNBC treatment.

Keywords

Dandelion (Taraxacum mongolicum Hand. -Mazz.) extract; Ferritinophagy; Ferroptosis; NCOA4; Triple negative breast cancer.

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