2. Academic Validation
  3. Involvement of AKT/β-catenin in Enhanced Osteogenesis by Cannabidiol in Bone Stromal Cells Harvested from Human Tori

Involvement of AKT/β-catenin in Enhanced Osteogenesis by Cannabidiol in Bone Stromal Cells Harvested from Human Tori

  • Sci Rep. 2026 Feb 20;16(1):9562. doi: 10.1038/s41598-025-03763-5.
Anupong Makeudom 1,  Mananchaya Taechukorn 1,  Kanyanut Muenhong 1,  Pantira Danvirunhavanit 1,  Janthima Petchnuan 1,  Ekapong Dechtham 1,  Pansakorn Sangkaew 1,  Sumit Suamphan 2,  Suttichai Krisanaprakornkit 3 4
Affiliations

Affiliations

  • 1 School of Dentistry, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
  • 2 Orthodontic Section, Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, 90112, Songkhla, Thailand.
  • 3 School of Dentistry, Mae Fah Luang University, Chiang Rai, 57100, Thailand. [email protected].
  • 4 School of Dentistry, Mae Fah Luang University Medical Center, Mae Fah Luang University, 365 Moo 12 Nang Lae Subdistrict, Mueang Chiang Rai District, Chiang Rai, 57100, Thailand. [email protected].
PMID: 41720800 DOI: 10.1038/s41598-025-03763-5
Abstract

Cannabidiol (CBD), a major cannabinoid of Cannabis sativa, has been utilized for several medical purposes, such as, an anti-resorptive activity that may benefit treatment of alveolar bone destruction in periodontitis. This study aimed to investigate osteogenic effects of CBD in primary bone stromal cells harvested from jaw tori of healthy patients. To determine cytotoxicity of CBD, the bone stromal cells were treated with various doses of CBD for 24 or 48 h and then analyzed by an alamarBlue® assay. No cytotoxicity was found in these cells treated with CBD up to 10 µM. Enhanced osteogenic differentiation and biomineralization of the cells treated with non-toxic doses of CBD were determined by Alkaline Phosphatase staining and Alizarin Red and von Kossa staining, respectively, and confirmed by upregulated mRNA expressions of runt-related transcription factor 2 (RUNX2), bone sialoprotein (BSP), and Osterix. Treatment with CBD significantly enhanced osteogenic differentiation and biomineralization and upregulated mRNA expressions of RUNX2, BSP, and Osterix (p < 0.05). Pretreatment with MK-2206, an Akt Inhibitor, or with CWP232228, a β-catenin antagonist, significantly decreased the increased Alizarin Red staining and the upregulated expressions of BSP and Osterix (p < 0.05), suggesting involvement of Akt/β-catenin in osteogenic induction upon treatment with CBD.

Keywords

Ak strain transforming (AKT); Cannabidiol; Osteoblast; Osteogenesis; β-catenin.

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