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  3. Biomimetic recombinant collagen with multicopy dual-targeted hemostatic motifs for enhanced hemostasis in surgical trauma

Biomimetic recombinant collagen with multicopy dual-targeted hemostatic motifs for enhanced hemostasis in surgical trauma

  • Trends Biotechnol. 2026 Mar 18:S0167-7799(26)00037-5. doi: 10.1016/j.tibtech.2026.01.017.
Zikun Zhang 1,  Xiaoxuan Ma 1,  Zhuo Zhang 2,  Weigang Yuwen 3,  Zhiguang Duan 1,  Rongzhan Fu 1,  Yang Li 4,  Chenhui Zhu 5,  Daidi Fan 6
Affiliations

Affiliations

  • 1 Engineering Research Center of Western Resource Innovation Medicine and Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Biotech. and Biomed. Research Institute, Northwest University, Xi'an 710127, China; Xi'an Synthetic Biology Technology and Biomaterials International Science and Technology Cooperation Base, School of Chemical Engineering, Northwest University, Xi'an 710127, China.
  • 2 Plastic and Cosmetic Maxillofacial Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710065, China.
  • 3 Xi'an Giant Biotechnology Co., Ltd, Xi'an 710065, China.
  • 4 Engineering Research Center of Western Resource Innovation Medicine and Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Biotech. and Biomed. Research Institute, Northwest University, Xi'an 710127, China; Xi'an Synthetic Biology Technology and Biomaterials International Science and Technology Cooperation Base, School of Chemical Engineering, Northwest University, Xi'an 710127, China. Electronic address: [email protected].
  • 5 Engineering Research Center of Western Resource Innovation Medicine and Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Biotech. and Biomed. Research Institute, Northwest University, Xi'an 710127, China; Xi'an Synthetic Biology Technology and Biomaterials International Science and Technology Cooperation Base, School of Chemical Engineering, Northwest University, Xi'an 710127, China. Electronic address: [email protected].
  • 6 Engineering Research Center of Western Resource Innovation Medicine and Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Biotech. and Biomed. Research Institute, Northwest University, Xi'an 710127, China; Xi'an Synthetic Biology Technology and Biomaterials International Science and Technology Cooperation Base, School of Chemical Engineering, Northwest University, Xi'an 710127, China. Electronic address: [email protected].
PMID: 41856809 DOI: 10.1016/j.tibtech.2026.01.017
Abstract

Collagen has superior bioactivity in hemostasis but is limited by a few active Amino acids and noncustomizable motifs. Here, we propose a biosynthetic strategy to construct multicopy dual-targeted recombinant Collagen. Using human Collagen type III alpha 1 chain (COL3α1) as a template, we integrated hemostatic motifs GFPGER and GPP, which bind Integrin α2β1 and Glycoprotein VI to enhance platelet adhesion, activation, and red blood cell (RBC) capture. The multicopy design further improves binding affinity, stability, and hemostatic activity. The recombinant Collagen significantly promotes platelet and RBC adhesion, platelet activation, fibrin network formation and exhibits good biocompatibility. In rat liver, spleen, and femoral artery bleeding models, it markedly reduces blood loss and bleeding time. Proteomic, molecular docking, and surface plasmon resonance analyses confirm motif-target interactions and activation of both primary and secondary hemostasis. This biomimetic recombinant Collagen shows strong potential as a hemostatic raw material and provides a strategy for functional Collagen synthesis.

Keywords

biomimetic recombinant collagen; biosynthesis; coagulation mechanisms; dual targeted; multicopy sequence.

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