2. Academic Validation
  3. ERAD inhibitor Eeyarestatin I (EerI) suppresses duck plague virus by restoring STING-mediated antiviral immunity

ERAD inhibitor Eeyarestatin I (EerI) suppresses duck plague virus by restoring STING-mediated antiviral immunity

  • Poult Sci. 2026 Jun;105(6):106808. doi: 10.1016/j.psj.2026.106808.
Yanming Tian 1,  Bin Tian 1,  Zaihui Liu 1,  Dongjie Cai 2,  Zhen Xiao 1,  Mingshu Wang 1,  Ying Wu 1,  Qiao Yang 1,  Shaqiu Zhang 1,  Dekang Zhu 3,  Mafeng Liu 1,  Xinxin Zhao 1,  Di Sun 1,  Juan Huang 1,  Xumin Ou 1,  Zhen Wu 1,  Yu He 1,  Yuhong Pan 1,  Renyong Jia 1,  Shun Chen 1,  Anchun Cheng 4
Affiliations

Affiliations

  • 1 Engineering Research Center of Southwest Animal Disease Prevention and Control Technology for Ministry of Education of the People's Republic of China, International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Research Center of Avian Disease and Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
  • 2 Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
  • 3 Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
  • 4 Engineering Research Center of Southwest Animal Disease Prevention and Control Technology for Ministry of Education of the People's Republic of China, International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Research Center of Avian Disease and Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Institute of Veterinary Immunology and Green Drugs, Veterinary Department in College of Aminal Science, State Key Laboratory of Green Pesticide, Guizhou. Electronic address: [email protected].
PMID: 41865653 DOI: 10.1016/j.psj.2026.106808
Abstract

Duck plague (DP), caused by the duck plague virus (DPV), is a highly contagious disease characterized by high morbidity and mortality, causing significant economic losses and biosecurity risks to the duck farming industry. Since current vaccines do not provide complete protection, the development of Antiviral drugs is urgently needed. In this study, we evaluated the anti-DPV activity of the ER-associated degradation (ERAD) pathway inhibitor Eeyarestatin I (EerI) in vitro. Our results demonstrated that EerI suppressed DPV proliferation in duck embryo fibroblast (DEF) cells in a dose-dependent manner, with the Antiviral effect persisting for over 96 hours. Further investigation revealed that EerI inhibits viral genome replication and cell-to-cell spread, thereby exerting a therapeutic Antiviral effect. Mechanistically, DPV Infection activates endoplasmic reticulum stress (ERS) and the ERAD pathway, promoting the degradation of STING via ERAD to suppress Antiviral immunity. EerI counteracts this process by blocking the ERAD pathway, thereby restoring STING stability and function and ultimately inhibiting DPV Infection. In conclusion, EerI is a candidate Antiviral agent against DPV.

Keywords

Antiviral agent; DPV; ERAD; Eeyarestatin I (EerI); STING.

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