2. Academic Validation
  3. Dynorphin A(1-8) in human placenta: amino acid sequence determined by tandem mass spectrometry

Dynorphin A(1-8) in human placenta: amino acid sequence determined by tandem mass spectrometry

  • Peptides. 1995;16(4):623-7. doi: 10.1016/0196-9781(95)00013-a.
A Agbas 1,  M S Ahmed,  W Millington,  B Cemerikic,  D M Desiderio,  J L Tseng,  C Dass
Affiliations

Affiliation

  • 1 School of Biological Sciences, Division of Molecular Biology and Biochemistry, University of Missouri, Kansas City 64108-2792, USA.
PMID: 7479294 DOI: 10.1016/0196-9781(95)00013-a
Abstract

Presence of the kappa receptor-preferring neuropeptide dynorphin A(1-8) in human placenta has been demonstrated by mass spectrometry to establish rigorously the appropriate molecular weight and amino acid sequence. Liquid secondary ionization mass spectrometry produced the protonated molecule ion, (M + H)+, at m/z 981 of the endogenous peptide, and tandem mass spectrometry collected the product ion spectrum that contained the appropriate amino acid sequence-determining fragment ions produced from the precursor ion (M + H)+. The amino acid sequence of the peptide is YGGFLRRI.

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