MEGA-8 (Synonyms: N-Octanoyl-N-methylglucamine)

製品番号: HY-W040218 純度: 99.33%: Data Sheet SDS COA 取扱説明書
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MEGA-8 (N-Octanoyl-N-methylglucamine) is a nonionic sugar-based surfactant. MEGA-8 can gently solubilize membrane proteins without easily causing their denaturation. MEGA-8 is a commonly used reagent for the extraction and study of membrane proteins in biochemistry.

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MEGA-8 構造式

CAS 番号 : 85316-98-9

容量		在庫状況
MOQ		Minimum order quantity
50 mg		お問い合わせ
100 mg		お問い合わせ
250 mg		お問い合わせ
Other size		お問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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•関連タンパク質:

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

体外実験

MEGA-8 can extract membrane-bound β-aryl ether lyase from Pseudomonas paucimobilis SYK-6 without disrupting its tertiary structure, and it can also detect the activity of β-aryl ether lyase in cell-free lysate^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

321.41

分子式

C₁₅H₃₁NO₆

CAS 番号

85316-98-9

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H](CN(C)C(CCCCCCC)=O)[C@@H](O)[C@H](O)[C@H](O)CO

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 175 mg/mL (544.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (155.56 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1113 mL	15.5565 mL	31.1129 mL
5 mM	0.6223 mL	3.1113 mL	6.2226 mL
10 mM	0.3111 mL	1.5556 mL	3.1113 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.33%

Select Batch:

データシート (268 KB) SDS (393 KB)

COA (242 KB) HNMR (251 KB) LCMS (87 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Masai E, et al. Detection and localization of a new enzyme catalyzing the beta-aryl ether cleavage in the soil bacterium (Pseudomonas paucimobilis SYK-6). FEBS Lett. 1989;249(2):348-352.

[2]. Jeffrey GA, et al. The structures of 1-deoxy-(N-methyloctanamido)-D-glucitol (MEGA-8) and 1-deoxy-(N-methylundecanamido)-D-glucitol (MEGA-11). Acta Crystallogr B. 1989;45 ( Pt 4):447-452.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.1113 mL	15.5565 mL	31.1129 mL	77.7823 mL
	5 mM	0.6223 mL	3.1113 mL	6.2226 mL	15.5565 mL
	10 mM	0.3111 mL	1.5556 mL	3.1113 mL	7.7782 mL
	15 mM	0.2074 mL	1.0371 mL	2.0742 mL	5.1855 mL
	20 mM	0.1556 mL	0.7778 mL	1.5556 mL	3.8891 mL
	25 mM	0.1245 mL	0.6223 mL	1.2445 mL	3.1113 mL
	30 mM	0.1037 mL	0.5185 mL	1.0371 mL	2.5927 mL
	40 mM	0.0778 mL	0.3889 mL	0.7778 mL	1.9446 mL
	50 mM	0.0622 mL	0.3111 mL	0.6223 mL	1.5556 mL
	60 mM	0.0519 mL	0.2593 mL	0.5185 mL	1.2964 mL
	80 mM	0.0389 mL	0.1945 mL	0.3889 mL	0.9723 mL
	100 mM	0.0311 mL	0.1556 mL	0.3111 mL	0.7778 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

MEGA-8 Related Classifications

Others

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MEGA-885316-98-9N-Octanoyl-N-methylglucamineMEGA8MEGA 8Biochemical Assay Reagentsglucitol chaincell-free lysateshydrogen-bond chainmembrane protein crystallizationorthorhombic crystalsmembrane-associated enzymeβ-aryl ether cleaving enzymeβ-hydroxypropiovanillonePseudomonas paucimobilis SYK-6Inhibitorinhibitorinhibit

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