MitoTam bromide, hydrobromide
MitoTam bromide, hydrobromide, a Tamoxifen derivative, is an electron transport chain (ETC) inhibitor. MitoTam bromide, hydrobromide reduces mitochondrial membrane potential in senescent cells and affects mitochondrial morphology. MitoTam bromide, hydrobromide is an effective anticancer agent, suppresses respiratory complexes (CI-respiration) and disrupts respiratory supercomplexes (SCs) formation in breast cancer cells.
For research use only. We do not sell to patients.
- CAS No.: 1634624-73-9
- Formula: C52H60Br2NOP
- Molecular Weight:905.82
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Storage:
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
MitoTam (0.5 μM-56 μM; 24 hours) kills breast cancer cell Lines and nonmalignant cells with an IC50 range from 0.65 μM to 55.9 μM[1].MitoTam (2.5 μM; 2-24 hours) results in stronger activation of the apoptotic pathway in MCF7 Her2 high cells compared with mock MCF7 cells[1].MitoTam (0.05 μM-1 μM; 3 days) causes a concentration-dependent induction of apoptosis in breast cancer cells, while there was no effect for non-malignant breast epithelial cells[2]
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Breast Cancer Cell Lines: BT474, MCF7, MCF7 Her2high, MCF7 Her2low, MDA-MB-231, MDA-MB-436, MDA-MB-453, SK-BR-3, T47D; NeuTL cells; Nonmalignant Cells: A014578, H9c2 cells
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Concentration:0.5 μM-56 μM
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Incubation Time:24 hours
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Result:Killed breast cancer cells MCF7, MCF7 Her2high, MCF7 Her2low with IC50 values of 1.25 μM, 0.65 μM and 1.45 μM respectively.
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Cell Line:MCF7 mock cells, MCF7 Her2high cells
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Concentration:2.5 μM
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Incubation Time:2 hours, 4 hours, 8 hours, 16 hours, 24 hours
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Result:Revealed accelerated cleavage of procaspase-9, Parp1/2 and proapoptotic Bax, decreased the antiapoptotic Bcl-2 protein in Her2high cells.
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Cell Line:MCF-7 cells, 4T1 cells and MCF-10a cells
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Concentration:0.05 μM-1 μM
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Incubation Time:3 days
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Result:Resulted in apoptosis in MCF7 and 4T1 cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:18-month-old or 2-month-old FVB/N mice[2]
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Dosage:2 μg/g
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Administration:Intraperitoneal injection; 2 μg/g; once a week; 4 weeks
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Result:Eliminated senescent cells also in vivo.
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Animal Model:FVB/N c-neu mouse[1]
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Dosage:0.54 μmol/mouse
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Administration:Intraperitoneal injection; 0.54 μmol/mouse; twice a week; 2 weeks
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Result:Suppressed Her2high breast carcinomas.
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Animal Model:Balb/c nude mice with MCF7 mock or MCF7 Her2high cells[1]
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Dosage:0.25 μmol/mouse/dose
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Administration:Intraperitoneal injection; 0.25 μmol/mouse/dose; twice a week; 2 weeks
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Result:Prevented reaching the ethical endpoint in all situations, slowed down the growth of MCF7 mock tumors and suppressed Her2high carcinomas decreased.
Chemical Information
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CAS No. 1634624-73-9
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Appearance Solid
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Molecular Weight 905.82
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Formula C52H60Br2NOP
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Color White to off-white
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SMILES
CN(CCOC1=CC=C(/C(C2=CC=CC=C2)=C(C3=CC=CC=C3)/CCCCCCCCCC[P+](C4=CC=CC=C4)(C5=CC=CC=C5)C6=CC=CC=C6)C=C1)C.Br[H].[Br-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Solvent & Solubility
DMSO : 240 mg/mL (264.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Rohlenova K, et al. Selective Disruption of Respiratory Supercomplexes as a New Strategy to Suppress Her2highBreast Cancer. Antioxid Redox Signal. 2017 Jan 10;26(2):84-103. [Content Brief]
[2]. Hubackova S, et al. Selective elimination of senescent cells by mitochondrial targeting is regulated by ANT2. Cell Death Differ. 2019 Jan;26(2):276-290. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1040 mL | 5.5199 mL | 11.0397 mL | 27.5993 mL |
| 5 mM | 0.2208 mL | 1.1040 mL | 2.2079 mL | 5.5199 mL | |
| 10 mM | 0.1104 mL | 0.5520 mL | 1.1040 mL | 2.7599 mL | |
| 15 mM | 0.0736 mL | 0.3680 mL | 0.7360 mL | 1.8400 mL | |
| 20 mM | 0.0552 mL | 0.2760 mL | 0.5520 mL | 1.3800 mL | |
| 25 mM | 0.0442 mL | 0.2208 mL | 0.4416 mL | 1.1040 mL | |
| 30 mM | 0.0368 mL | 0.1840 mL | 0.3680 mL | 0.9200 mL | |
| 40 mM | 0.0276 mL | 0.1380 mL | 0.2760 mL | 0.6900 mL | |
| 50 mM | 0.0221 mL | 0.1104 mL | 0.2208 mL | 0.5520 mL | |
| 60 mM | 0.0184 mL | 0.0920 mL | 0.1840 mL | 0.4600 mL | |
| 80 mM | 0.0138 mL | 0.0690 mL | 0.1380 mL | 0.3450 mL | |
| 100 mM | 0.0110 mL | 0.0552 mL | 0.1104 mL | 0.2760 mL |
MitoTam bromide, hydrobromide Related Classifications
HY-126222 Related Classifications
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