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NBD-PE 

Cat. No.: HY-D1683
Handling Instructions

NBD-PE is an effective lipid fluorescent probe (Excitation/Emission: 465/535 nm; Color: Green). NBD-PE offers a wide array of applications in membrane and cell biology.

For research use only. We do not sell to patients.

NBD-PE Chemical Structure

NBD-PE Chemical Structure

CAS No. : 178119-00-1

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Description

NBD-PE is an effective lipid fluorescent probe (Excitation/Emission: 465/535 nm; Color: Green). NBD-PE offers a wide array of applications in membrane and cell biology[1][2][3].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Assay of phospholipid transfer activity[3]:
1. The reaction mixture containes 2 mg mitochondrial protein, 0.02 μmol of NBD-PE, 0.5 mmol of Tris-HCl, pH 7.4, and 0-200 μg of pH 5.l-supernatant protein.
2. Samples are incubated at 37°C for 1 hour with gentle agitation.
3. The reaction is terminated by placing samples in an ice bath for 10 min, followed by centrifugation at 15,000 g for 5 min.
4. The supernatants are decanted, allowed to come to room temperature, and the absorbance (460 nm) and the relative fluorescence at 535 nm (excitation at 465 nm) are determined.
5. Blanks containing only NBD-PE and exchange protein are run concomitantly to correct for possible interaction between NBD-PE and exchange protein.
6. The percent exchange of NBD-PE is calculated from the differences between the absorbances or relative fluorescence in the supernatant fractions in the presence and absence of exchange protein.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

956.24

Formula

C49H90N5O11P

CAS No.
SMILES

CCN(CC)CC.CCCCCCCCCCCCCCCC(O[[email protected]](COC(CCCCCCCCCCCCCCC)=O)COP(OCCNC1=CC=C([N+]([O-])=O)C2=NON=C12)(O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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NBD-PE
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HY-D1683
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